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PMID: 3837182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes.

Molecular and cellular biology ·Vol. 5 ·No. 10 ·1985-10-00 ·Pages 2720-32

Ng SY, Gunning P, Eddy R, Ponte P, Leavitt J, Shows T, Kedes L

Abstract

We have assigned six members of the human beta-actin multigene family to specific human chromosomes. The functional gene, ACTB, is located on human chromosome 7, and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. Using intervening sequence probes, we showed that the functional gene is single copy and that all of the other beta-actin related sequences are recently generated in evolution and are probably processed pseudogenes. The entire nucleotide sequence of the functional gene has been determined and is identical to cDNA clones in the coding and 5' untranslated regions. We have previously reported that the 3' untranslated region is well conserved between humans and rats (Ponte et al., Nucleic Acids Res. 12:1687-1696, 1984). Now we report that four additional noncoding regions are evolutionarily conserved, including segments of the 5' flanking region, 5' untranslated region, and, surprisingly, intervening sequences I and III. These conserved sequences, especially those found in the introns, suggest a role for internal sequences in the regulation of beta-actin gene expression.

MeSH Terms
Actins/genetics Animals Base Sequence Chickens Chromosome Mapping DNA/genetics Enhancer Elements, Genetic Genes Humans Nucleic Acid Conformation Promoter Regions, Genetic RNA, Messenger/genetics Sequence Homology, Nucleic Acid
Chemicals
Actins RNA, Messenger DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ng S Y
Gunning P
Eddy R
Ponte P
Leavitt J
Shows T
Kedes L
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55 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.10.2720-2732.1985
Published
1985-10-00
Pages
2720-32
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367010
Subset
IM
Grants
NCI NIH HHS · CA34763 · United States
NIGMS NIH HHS · GM20454 · United States
NICHD NIH HHS · HD17031 · United States
Databases
GENBANK
J00074, M10278, X00351
External Links
PubMed source DOI: 10.1128/mcb.5.10.2720-2732.1985 PMC Full Text View journal details
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