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PMID: 6334230 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription control region within the protein-coding portion of adenovirus E1A genes.

Molecular and cellular biology ·Vol. 4 ·No. 7 ·1984-07-00 ·Pages 1293-305

Osborne TF, Arvidson DN, Tyau ES, Dunsworth-Browne M, Berk AJ

Abstract

A single-base deletion within the protein-coding region of the adenovirus type 5 early region 1A (E1A) genes, 399 bases downstream from the transcription start site, depresses transcription to 2% of the wild-type rate. Complementation studies demonstrated that this was due to two effects of the mutation: first, inactivation of an E1A protein, causing a reduction by a factor of 5; second, a defect which acts in cis to depress E1A mRNA and nuclear RNA concentrations by a factor of 10. A larger deletion within the protein-coding region of E1A which overlaps the single-base deletion produces the same phenotype. In contrast, a linker insertion which results in a similar truncated E1A protein does not produce the cis-acting defect in E1A transcription. These results demonstrate that a critical cis-acting transcription control region occurs within the protein coding sequence in adenovirus type 5 E1A. The single-base deletion occurs in a sequence which shows extensive homology with a sequence from the enhancer regions of simian virus 40 and polyomavirus. This region is not required for E1A transcription during the late phase of infection.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Cell Nucleus/physiology Cell Transformation, Viral Genes Genes, Viral HeLa Cells/physiology Humans Mutation Nucleic Acid Hybridization Plasmids RNA, Messenger/genetics RNA, Neoplasm/genetics RNA, Viral/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
RNA, Messenger RNA, Neoplasm RNA, Viral Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Osborne T F
Arvidson D N
Tyau E S
Dunsworth-Browne M
Berk A J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.7.1293-1305.1984
Published
1984-07-00
Pages
1293-305
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368911
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.4.7.1293-1305.1984 PMC Full Text View journal details
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