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PMID: 291030 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An adenovirus type 5 early gene function regulates expression of other early viral genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 8 ·1979-08-00 ·Pages 3665-9

Jones N, Shenk T

Abstract

We have identified an adenovirus type 5(Ad5) early gene function located in early region 1 which is required for the production of early cytoplasmic mRNAs corresponding to early regions 2, 3, and 4. Mutant dl312 (lacks the segment between 1.5 and 4.5 map units) grows as well as wild-type virus in 293 cells (Ad5-transformed human embryonic kidney cells), but its growth is severely restricted in HeLa cells. We detect no viral RNAs in the cytoplasm of dl312-infected HeLa cells. Viral RNA sequences are present, however, in dl312-infected HeLa cell nuclei.

MeSH Terms
Adenoviruses, Human/genetics Chromosome Deletion Genes Genes, Viral Mutation RNA, Messenger/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
RNA, Messenger Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jones N
Shenk T
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.8.3665
Published
1979-08-00
Pages
3665-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383893
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.8.3665 PMC Full Text View journal details
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