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PMID: 6334309 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Specific stimulation of actin gene transcription by epidermal growth factor and cycloheximide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 23 ·1984-12-00 ·Pages 7476-80

Elder PK, Schmidt LJ, Ono T, Getz MJ

Abstract

Stimulation of quiescent AKR-2B mouse embryo cells with epidermal growth factor (EGF) results in a rapid and specific induction of actin mRNA sequences. These mRNAs include those coding for both beta- and gamma-cytoskeletal, but not alpha-skeletal muscle, actin isotypes. Elongation of nascent RNA chains in isolated nuclei (run-off transcription) demonstrates that the mRNA accumulation is preceded by an increase in actin gene transcription. This increase is transient, however, and is followed by a rapid attenuation of transcriptional activity. An inhibitor of protein synthesis, cycloheximide, was also found to induce beta- and gamma-actin mRNA accumulation. Furthermore, the simultaneous addition of EGF and cycloheximide produced a synergistic effect on actin sequences in both steady-state nuclear and polysomal RNA. Run-off transcription experiments demonstrate that this synergistic effect results from an increase in the magnitude and duration of actin gene transcription. It is also specific in that alpha-tubulin gene transcription is not similarly affected. These data suggest the existence of a specific labile repressor of actin gene transcription.

MeSH Terms
Actins/genetics Animals Cell Line Cycloheximide/pharmacology Embryo, Mammalian Epidermal Growth Factor/pharmacology Genes/drug effects Mice Mice, Inbred AKR RNA, Messenger/genetics,isolation & purification Transcription, Genetic/drug effects
Chemicals
Actins RNA, Messenger Epidermal Growth Factor Cycloheximide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Elder P K
Schmidt L J
Ono T
Getz M J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.23.7476
Published
1984-12-00
Pages
7476-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392169
Subset
IM
Grants
NCI NIH HHS · CA33643 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.23.7476 PMC Full Text View journal details
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