Home LiteratureArticle Details
PMID: 6576337 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Growth-related changes in specific mRNAs of cultured mouse cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 14 ·1983-07-00 ·Pages 4271-5

Linzer DI, Nathans D

Abstract

A cDNA plasmid library has been constructed from the poly(A)+ RNA present in BALB/c3T3 cells after serum stimulation. Of 3,500 clones tested, approximately 0.5% contained inserts corresponding to mRNAs present at higher levels in serum-stimulated BALB/c 3T3 cell cultures than in quiescent cultures. Most of these RNA species increased 2- to 5-fold, and the kinetics of increase for various RNAs differed. One clone (28H6) hybridized to a 1-kilobase RNA species that is present at barely detectable levels in resting cells but is increased at least 15- to 20-fold after serum stimulation, reaching a maximal level coincident with the onset of DNA synthesis. This RNA was at a high level in proliferating cells but decreased rapidly as cells reached confluence. 28H6 RNA was also increased in resting cells infected with simian virus 40 or stimulated with platelet-derived growth factor.

MeSH Terms
Animals Cell Division Cells, Cultured Clone Cells Cloning, Molecular DNA/analysis Kinetics Mice Mice, Inbred BALB C Mitogens/pharmacology Nucleic Acid Hybridization Plasmids RNA, Messenger/genetics
Chemicals
Mitogens RNA, Messenger DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Linzer D I
Nathans D
References (40)
40 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. SV40 large tumor antigen can regulate some cellular transcripts in a positive fashion.
    Cell. 1982 Sep;30(2):481-90 PMID: 6291771
  3. Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3082-6 PMID: 6179076
  4. The initiation of cell division in a contact-inhibited mammalian cell line.
    J Cell Physiol. 1965 Dec;66(3):325-33 PMID: 5884360
  5. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  6. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  7. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  8. Control of translation in cultured cells: continued synthesis and accumulation of messenger RNA in nondividing cultures.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):750-4 PMID: 4207072
  9. Animal cell cycle.
    Annu Rev Biochem. 1978;47:715-50 PMID: 354504
  10. Changes in RNA in relation to growth of the fibroblast. III. Posttranscriptional regulation of mRNA formation in resting and growing cells.
    Cell. 1975 Jan;4(1):69-75 PMID: 1078787
  11. Polyadenylylated RNA complementary to a mouse retrovirus-like multigene family is rapidly and specifically induced by epidermal growth factor stimulation of quiescent cells.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7317-21 PMID: 6296829
  12. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
    FEBS Lett. 1977 Oct 15;82(2):314-6 PMID: 913604
  13. Growth control in cultured mouse fibroblasts: induction of the pleiotypic and mitogenic responses by a purified growth factor.
    Proc Natl Acad Sci U S A. 1974 Jul;71(7):2600-4 PMID: 4527822
  14. The messenger RNA sequences in growing and resting mouse fibroblasts.
    Cell. 1975 Oct;6(2):197-206 PMID: 1182801
  15. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  16. Platelet-derived growth factor. I. High yield purification and evidence for multiple forms.
    J Biol Chem. 1982 May 10;257(9):5154-60 PMID: 7068680
  17. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
    J Bacteriol. 1974 Sep;119(3):1072-4 PMID: 4605400
  18. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  19. Platelet-derived growth factor-modulated proteins: constitutive synthesis by a transformed cell line.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4358-62 PMID: 6170062
  20. Transport changes rapidly initiated by serum addition to "contact inhibited" 3T3 cells.
    Proc Natl Acad Sci U S A. 1969 Nov;64(3):1049-56 PMID: 4313331
  21. Platelets as a source of fibroblast growth-promoting activity.
    Exp Cell Res. 1974 Aug;87(2):297-301 PMID: 4370268
  22. The platelet-derived growth factor.
    Cell. 1978 Jun;14(2):203-10 PMID: 352535
  23. Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794-8 PMID: 6777776
  24. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  25. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.
    J Cell Biol. 1963 May;17:299-313 PMID: 13985244
  26. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
    J Biol Chem. 1978 Apr 10;253(7):2483-95 PMID: 75887
  27. Recovery of DNA fragments inserted by the "tailing" method: regeneration of PstI restriction sites.
    Gene. 1981 May;13(4):339-46 PMID: 6266921
  28. CONTACT INHIBITION, MACROMOLECULAR SYNTHESIS, AND POLYRIBOSOMES IN CULTURED HUMAN DIPLOID FIBROBLASTS.
    Proc Natl Acad Sci U S A. 1965 Feb;53:350-6 PMID: 14294068
  29. Regulation of RNA synthesis in fibroblasts during transition from resting to growing state.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2819-22 PMID: 4517934
  30. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  31. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
    Nucleic Acids Res. 1976 Jan;3(1):101-16 PMID: 765970
  32. Control of macromolecular synthesis in proliferating and resting Syrian hamster cells in monolayer culture. I. Ribosome function.
    J Cell Physiol. 1971 Feb;77(1):31-42 PMID: 5546177
  33. DNA restriction enzyme from E. coli.
    Nature. 1968 Mar 23;217(5134):1110-4 PMID: 4868368
  34. Homology between mammalian cell DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high-complexity probe.
    Cell. 1982 Nov;31(1):71-80 PMID: 6297753
  35. Transcriptional and translational control of cytoplasmic proteins after serum stimulation of quiescent Swiss 3T3 cells.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5712-6 PMID: 6946510
  36. The induction of cellular DNA synthesis by simian virus 40 in contact-inhibited and in x-irradiated cells.
    Proc Natl Acad Sci U S A. 1966 Sep;56(3):918-25 PMID: 16578647
  37. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  38. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
    Cell. 1980 May;20(1):95-105 PMID: 6893015
  39. Control of the initiation of DNA synthesis in 3T3 cells: low-molecular weight nutrients.
    Proc Natl Acad Sci U S A. 1974 Aug;71(8):2942-5 PMID: 4528490
  40. "Pleiotypic response".
    Nat New Biol. 1971 Aug;232(33):206-11 PMID: 5285753
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.14.4271
Published
1983-07-00
Pages
4271-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384019
Subset
IM
Grants
NCI NIH HHS · 1 F32 CA 06699 · United States
NCI NIH HHS · 5 P01 CA 16519 · United States
NIGMS NIH HHS · GM 27765 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.14.4271 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2