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PMID: 16453808 Published · ppublish English Journal Article

5' Analysis of the soybean leghaemoglobin lbc(3) gene: regulatory elements required for promoter activity and organ specificity.

The EMBO journal ·Vol. 6 ·No. 12 ·1987-12-01 ·Pages 3565-9

Stougaard J, Sandal NN, Grøn A, Kühle A, Marcker KA

Abstract

The soybean leghaemoglobin lbc(3) gene promoter was analysed in transgenic Lotus corniculatus plants. Hybrid-promoter constructions and 5' deletions were studied using chimeric genes composed of the various promoters, the chloramphenicol acetyltransferase (CAT) coding sequence and the lbc(3) 3' flanking region. A 5' Bal31 deletion series mapped a strong positive regulatory element between -1100 and -950. A weaker element located between -230 and -170 defined the minimum 5' region required for detectable promoter activity. Reactivation of inactive promoters with deletion endpoints between -230 and the transcription initiation site was obtained employing the constitutive cauliflower mosaic virus (CaMV) 35S enhancer. The position of cis regulatory element(s) required for nodule-specific expression was defined to 37 bp between -139 and -102. This region contains sequences conserved in other leghaemoglobin and nodulin genes. No indispensable control elements were found on the lbc(3) 3' flanking region.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stougaard J
Department of Molecular Biology and Plant Physiology, University of Aarhus, C. F. Møllers Allé 130, DK-8000 Aarhus C, Denmark.
Sandal N N
Grøn A
Kühle A
Marcker K A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-12-01
Pages
3565-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553822
External Links
PubMed source PMC Full Text View journal details
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