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PMID: 6208532 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A universal model for the secondary structure of 5.8S ribosomal RNA molecules, their contact sites with 28S ribosomal RNAs, and their prokaryotic equivalent.

Nucleic acids research ·Vol. 12 ·No. 19 ·1984-10-11 ·Pages 7479-502

Vaughn JC, Sperbeck SJ, Ramsey WJ, Lawrence CB

Abstract

The phylogenetic approach (ref. 1) has been utilized in construction of a universal 5.8S rRNA secondary structure model, in which about 65% of the residues exist in paired structures. Conserved nucleotides primarily occupy unpaired regions. Multiple compensating base changes are demonstrated to be present in each of the five postulated helices, thereby forming a major basis for their proof. The results of chemical and enzymatic probing of 5.8S rRNAs (ref. 13, 32) are fully consistent with, and support, our model. This model differs in several ways from recently proposed 5.8S rRNA models (ref. 3, 4), which are discussed. Each of the helices in our model has been extended to the corresponding bacterial, chloroplast and mitochondrial sequences, which are demonstrated to be positionally conserved by alignment with their eukaryotic counterparts. This extension is also made for the base paired 5.8S/28S contact points, and their prokaryotic and organelle counterparts. The demonstrated identity of secondary structure in these diverse molecules strongly suggests that they perform equivalent functions in prokaryotic and eukaryotic ribosomes.

MeSH Terms
Animals Base Sequence Eukaryotic Cells/ultrastructure Nucleic Acid Conformation Prokaryotic Cells/ultrastructure RNA, Bacterial RNA, Ribosomal Ribosomes/ultrastructure Thermodynamics
Chemicals
RNA, Bacterial RNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vaughn J C
Sperbeck S J
Ramsey W J
Lawrence C B
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51 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.19.7479
Published
1984-10-11
Pages
7479-502
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320176
Subset
IM
Grants
NIAID NIH HHS · AI-19578 · United States
External Links
PubMed source DOI: 10.1093/nar/12.19.7479 PMC Full Text View journal details
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