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PMID: 7088713 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Primary and secondary structure of 5.8S rRNA from the silkgland of Bombyx mori.

Nucleic acids research ·Vol. 10 ·No. 7 ·1982-04-10 ·Pages 2415-8

Fujiwara H, Kawata Y, Ishikawa H

Abstract

Nucleotide sequence of 5.8S rRNA of the silkworm, Bombyx mori has been determined by gel sequencing methods. The 5.8S rRNA was the longest so far reported, with the 5'-terminal sequence several nucleotides longer than those of the other organisms. Upon constructing the secondary structure in accordance with the "burp gun" model (12), the Bombyx 5.8S rRNA formed a wide-open "muzzle" due to several unpaired bases at the ends. The overall structure also appeared less stable with less G . C pairs and more unpaired bases than that of the HeLa 5.8S rRNA. These structural features may be essential for those 5.8S rRNAs which interact with 28S rRNAs containing the hidden break to form a stable complex.

MeSH Terms

Animals Base Sequence Bombyx/analysis Exocrine Glands/analysis Humans Molecular Weight Nucleic Acid Conformation RNA, Ribosomal Species Specificity

Chemicals

RNA, Ribosomal

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Fujiwara H

Kawata Y

Ishikawa H

References (13)

13 references, click to expand

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Biochem Biophys Res Commun. 1979 Sep 27;90(2):417-24 PMID: 508311
Sequence and secondary structure of Drosophila melanogaster 5.8S and 2S rRNAs and of the processing site between them.
Nucleic Acids Res. 1979 Dec 20;7(8):2213-38 PMID: 118436
Coding and spacer sequences in the 5.8S-2S region of Sciara coprophila ribosomal DNA.
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Effect of point mutations on 5.8S ribosomal ribonucleic acid secondary structure and the 5.8S--28S ribosomal ribonucleic acid junction.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.7.2415

Published

1982-04-10

Pages

2415-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320619

Subset

Databases

GENBANK

M10368

External Links

PubMed source DOI: 10.1093/nar/10.7.2415 PMC Full Text View journal details

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Primary and secondary structure of 5.8S rRNA from the silkgland of Bombyx mori.

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