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PMID: 6322119 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Nucleotide sequence of the 18S-26S rRNA intergene region of the sea urchin.

Nucleic acids research ·Vol. 12 ·No. 3 ·1984-02-10 ·Pages 1737-47

Hindenach BR, Stafford DW

Abstract

The DNA sequence which spans the internal transcribed spacers of a cloned ribosomal transcription unit from the sea urchin, Lytechinus variegatus, has been determined. The region extends from the conserved Eco RI site near the 3' end of the 18S rDNA to a Bam HI site in the 26S rDNA and includes 232 nucleotides coding for 18S rRNA, 367 nucleotides of internal transcribed spacer, 159 nucleotides coding for 5.8S rRNA, 338 nucleotides of internal transcribed spacer, and 505 nucleotides coding for 26S rRNA. The rRNA coding regions were identified by direct analysis of 3'-labeled 18S and 5.8S rRNA and 5'-labeled 5.8S rRNA, and by sequence homology of the 26S rDNA with yeast and vertebrate 26/28S rRNAs. The internal transcribed spacers are GC-rich, similar to those of vertebrates. The 5.8S and 5' 26S rDNA sequences support a proposed model for a structural domain of the yeast large subunit ribosomal RNA (Veldman et al. [1981] Nucleic Acids Res. 9, 6935-6952).

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Genes RNA, Ribosomal/genetics Ribosomes/metabolism Sea Urchins Species Specificity Transcription, Genetic
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hindenach B R
Stafford D W
References (27)
27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.3.1737
Published
1984-02-10
Pages
1737-47
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318612
Subset
IM
Databases
GENBANK
X00350
External Links
PubMed source DOI: 10.1093/nar/12.3.1737 PMC Full Text View journal details
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