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PMID: 6815618 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Primary and secondary structures of Tetrahymena and aphid 5.8S rRNAs: structural features of 5.8S rRNA which interacts with the 28S rRNA containing the hidden break.

Nucleic acids research ·Vol. 10 ·No. 17 ·1982-09-11 ·Pages 5173-82

Fujiwara H, Ishikawa H

Abstract

The Tetrahymena 5.8S rRNA is 154 nucleotides long, the shortest so far reported except for the split 5.8S rRNAs of Diptera (m5.8S plus 2S rRNA). In this molecule several nucleotides are deleted in the helix e (GC-rich stem) region. Upon constructing the secondary structure in accordance with "burp-gun" model, the Tetrahymena 5.8S rRNA forms a wide-open "muzzle" of the terminal regions due to both extra nucleotides and several unpaired bases. The aphid 5.8S rRNA consists of 161 nucleotides and can form stable helices in both terminal and helix e regions. As a whole, the secondary structure of Tetrahymena 5.8S rRNA resembles that of Bombyx 5.8S molecule while the aphid 5.8S rRNA shares several structural features with the HeLa 5.8S molecule. Likely, the 5.8S rRNA attached to the 28S rRNA with the hidden break differs in structure from those interacting with the 28S partners without the break. Nucleotide sequences of 5.8S rRNA in insects as well as in protozoans are not so conservative evolutionarily as in vertebrates.

MeSH Terms
Animals Aphids/genetics Base Sequence Bombyx/genetics Molecular Weight Nucleic Acid Conformation RNA, Ribosomal/genetics Species Specificity Tetrahymena pyriformis/genetics
Chemicals
RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fujiwara H
Ishikawa H
References (26)
26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.17.5173
Published
1982-09-11
Pages
5173-82
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320863
Subset
IM
Databases
GENBANK
M10751, M10752
External Links
PubMed source DOI: 10.1093/nar/10.17.5173 PMC Full Text View journal details
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