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PMID: 7079176 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of an exceptionally long 5.8S ribosomal RNA from Crithidia fasciculata.

Nucleic acids research ·Vol. 10 ·No. 6 ·1982-03-25 ·Pages 2085-92

Schnare MN, Gray MW

Abstract

In Crithidia fasciculata, a trypanosomatid protozoan, the large ribosomal subunit contains five small RNA species (e, f, g, i, j) in addition to 5S rRNA [Gray, M.W. (1981) Mol. Cell. Biol. 1, 347-357]. The complete primary sequence of species i is shown here to be pAACGUGUmCGCGAUGGAUGACUUGGCUUCCUAUCUCGUUGA ... AGAmACGCAGUAAAGUGCGAUAAGUGGUApsiCAAUUGmCAGAAUCAUUCAAUUACCGAAUCUUUGAACGAAACGG ... CGCAUGGGAGAAGCUCUUUUGAGUCAUCCCCGUGCAUGCCAUAUUCUCCAmGUGUCGAA(C)OH. This sequence establishes that species i is a 5.8S rRNA, despite its exceptional length (171-172 nucleotides). The extra nucleotides in C. fasciculata 5.8S rRNA are located in a region whose primary sequence and length are highly variable among 5.8S rRNAs, but which is capable of forming a stable hairpin loop structure (the "G+C-rich hairpin"). The sequence of C. fasciculata 5.8S rRNA is no more closely related to that of another protozoan, Acanthamoeba castellanii, than it is to representative 5.8S rRNA sequences from the other eukaryotic kingdoms, emphasizing the deep phylogenetic divisions that seem to exist within the Kingdom Protista.

MeSH Terms
Animals Base Sequence Crithidia/genetics Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Phylogeny RNA, Ribosomal Species Specificity
Chemicals
RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schnare M N
Gray M W
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.6.2085
Published
1982-03-25
Pages
2085-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320590
Subset
IM
Databases
GENBANK
X01521
External Links
PubMed source DOI: 10.1093/nar/10.6.2085 PMC Full Text View journal details
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