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PMID: 7433112 Published · ppublish English Comparative Study Journal Article

The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA.

Nucleic acids research ·Vol. 8 ·No. 19 ·1980-10-10 ·Pages 4365-76

Azad AA, Deacon NJ

Abstract

The 3'-terminal sequences of 18S rRNA from chicken reticulocyte, mouse sarcoma, rat liver, rabbit reticulocyte and barley embryo were determined by the direct chemical sequencing method. The regions sequenced show complete homology for the first 73 nucleotides. A sequence 5'-proximal to the m6(2)Am6(2)A residues that is complementary to eukaryotic 5S RNAs is totally conserved. This supports the hypothesis that base-paired interaction between 5S and 18S rRNA, which are present in the large and small ribosomal subunits respectively, may be involved in the reversible association of ribosomal subunits during protein synthesis.

MeSH Terms
Animals Base Composition Base Sequence Chickens Hordeum/analysis Liver/analysis Mice Molecular Weight Plants/analysis RNA, Ribosomal Rats Reticulocytes/analysis Sarcoma, Experimental/analysis Species Specificity
Chemicals
RNA, Ribosomal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Azad A A
Deacon N J
References (43)
43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.19.4365
Published
1980-10-10
Pages
4365-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324245
Subset
IM
Databases
GENBANK
J01875, K01379, K01380, K01381, K01382
External Links
PubMed source DOI: 10.1093/nar/8.19.4365 PMC Full Text View journal details
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