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PMID: 724498 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Reactions at the termini of tRNA with T4 RNA ligase.

Nucleic acids research ·Vol. 5 ·No. 10 ·1978-10-00 ·Pages 3665-77

Bruce AG, Uhlenbeck OC

Abstract

T4 RNA ligase will catalyze the addition of nucleoside 3', 5'-bisphosphates onto the 3' terminus of tRNA resulting in tRNA molecule one nucleotide longer with a 3' terminal phosphate. Under appropriate conditions the reaction is quantitative and, if high specific radioactivity bisphosphates are used, it provides an efficient means for in vitro labeling of tRNA. Although the 3' terminal hydroxyl is a good acceptor, the 5' terminal phosphate in most tRNA's is not an effective donor in the RNA ligase reaction. This poor reactivity is due to the secondary structure of the 5' terminal nucleotide. If E. Coli tRNAf Met is used, the 5' phosphate is reactive and the major product with RNA ligase is the cyclic tRNA.

MeSH Terms
Coliphages/enzymology Dimethyl Sulfoxide Kinetics Methionine Phenylalanine Polynucleotide Ligases/metabolism RNA Ligase (ATP)/metabolism RNA, Transfer/metabolism Substrate Specificity Temperature
Chemicals
Phenylalanine RNA, Transfer Methionine Polynucleotide Ligases RNA Ligase (ATP) Dimethyl Sulfoxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bruce A G
Uhlenbeck O C
References (24)
24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.10.3665
Published
1978-10-00
Pages
3665-77
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342702
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.10.3665 PMC Full Text View journal details
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