Home LiteratureArticle Details
PMID: 537919 Published · ppublish English Journal Article

Do eukaryotic mRNA 5' noncoding sequences base-pair with the 18 S ribosomal RNA 3' terminus?

Nucleic acids research ·Vol. 7 ·No. 7 ·1979-12-11 ·Pages 2045-54

De Wachter R

Abstract

Protein synthesis initiation on prokaryotic mRNAs involves base-pairing of a site preceding the initiation codon with the 3' terminal sequence of 16 S rRNA. It has been suggested that a similar situation may prevail in eukaryotic mRNAs. This suggestion is not based on experiments, but on observation of complementarities between mRNA 5' noncoding sequences and a conserved sequence near the 18 S rRNA 3' terminus. The hypothesis can be evaluated by comparing the number of potential binding sites found in the 5' noncoding sequences with the number of such sites expected to occur by chance. A method for computing this number is presented. The 5' noncoding sequences contain more binding sites than expected for a random RNA chain, but the same is true for 3' noncoding sequences. The effect can be traced to a clustering of purines and pyrimidines, common to noncoding sequences. In conclusion, a close inspection of the available mRNA sequences does not reveal any indication of a specific base-pairing ability between their 5' noncoding segments and the 18 S rRNA 3' terminus.

MeSH Terms
Animals Base Sequence Binding Sites Codon Humans RNA, Messenger/metabolism RNA, Ribosomal/metabolism
Chemicals
Codon RNA, Messenger RNA, Ribosomal
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
De Wachter R
References (35)
35 references, click to expand
  1. Rous sarcoma virus genome is terminally redundant: the 5' sequence.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):989-93 PMID: 66683
  2. Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.
    Cell. 1978 Mar;13(3):551-63 PMID: 77738
  3. Base repetitions and sequence repetitions in 3', noncoding sequences of eukaryotic messenger RNA's [proceedings].
    Arch Int Physiol Biochim. 1979 May;87(2):403-4 PMID: 92953
  4. DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli.
    Cell. 1978 Sep;15(1):215-29 PMID: 151587
  5. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  6. The 5'-terminal leader sequence of late 16 S mRNA from cells infected with simian virus 40.
    J Biol Chem. 1978 May 25;253(10):3643-7 PMID: 206548
  7. Complete sequences of the ribosome recognition sites in vesicular stomatitis virus mRNAs: recognition by the 40S and 80S complexes.
    Cell. 1978 Jun;14(2):345-53 PMID: 208778
  8. How do eucaryotic ribosomes select initiation regions in messenger RNA?
    Cell. 1978 Dec;15(4):1109-23 PMID: 215319
  9. Nucleotide sequence of cro, cII and part of the O gene in phage lambda DNA.
    Nature. 1978 Mar 30;272(5652):410-4 PMID: 264238
  10. Nucleotide sequence of 5' terminus of alfalfa mosaic virus RNA 4 leading into coat protein cistron.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5504-8 PMID: 271973
  11. The nucleotide sequence of the 5' untranslated region of human gamma-globin mRNA.
    Nucleic Acids Res. 1978 Oct;5(10):3515-22 PMID: 318162
  12. Sequence of the 3'-noncoding and adjacent coding regions of human gamma-globin mRNA.
    Nucleic Acids Res. 1978 Dec;5(12):4625-30 PMID: 318163
  13. Nucleotide sequence of the promoter--operator region of the tryptophan operon of Escherichia coli.
    J Mol Biol. 1978 May 15;121(2):113-37 PMID: 351191
  14. Sequence of the lacI gene.
    Nature. 1978 Aug 24;274(5673):765-9 PMID: 355891
  15. Nucleotide sequence of the L-arabinose regulatory region of Escherichia coli K12.
    J Biol Chem. 1978 Oct 10;253(19):6931-3 PMID: 357433
  16. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41 PMID: 358200
  17. Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4271-5 PMID: 360214
  18. DNA sequence from the histidine operon control region: seven histidine codons in a row.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4281-5 PMID: 360216
  19. The regulatory region of the biotin operon in Escherichia coli.
    Nature. 1978 Dec 14;276(5689):689-94 PMID: 366433
  20. Nucleotide sequence of the 5' terminus of satellite tobacco necrosis virus ribonucleic acid.
    Biochemistry. 1979 Apr 3;18(7):1361-6 PMID: 427118
  21. Complete nucleotide sequence of the 5' noncoding region of human alpha-and beta-globin mRNA.
    Cell. 1977 Dec;12(4):1085-95 PMID: 597858
  22. Nucleotide sequence at the 5' extremity of tobacco-mosaic-virus RNA. 1. The noncoding region (nucleotides 1-68).
    Eur J Biochem. 1978 Mar 15;84(2):513-9 PMID: 639800
  23. Sequence of chicken ovalbumin mRNA.
    Nature. 1978 Jun 29;273(5665):723-8 PMID: 661981
  24. Coincidence of the promoter and capped 5' terminus of RNA from the adenovirus 2 major late transcription unit.
    Cell. 1978 Dec;15(4):1463-75 PMID: 729003
  25. The nucleotide sequence of bacteriophage phiX174.
    J Mol Biol. 1978 Oct 25;125(2):225-46 PMID: 731693
  26. Nucleotide sequence of bacteriophage fd DNA.
    Nucleic Acids Res. 1978 Dec;5(12):4495-503 PMID: 745987
  27. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  28. A-protein gene of bacteriophage MS2.
    Nature. 1975 Jul 24;256(5515):273-8 PMID: 806810
  29. Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes.
    Nature. 1975 Aug 21;256(5519):624-8 PMID: 807854
  30. Completed DNA sequences and organization of repressor-binding sites in the operators of phage lambda.
    J Mol Biol. 1977 May 15;112(2):265-77 PMID: 875019
  31. An intercistronic region and ribosome-binding site in bacterial messenger RNA.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2399-403 PMID: 1094468
  32. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
  33. E. coli lactose operon ribosome binding site.
    Nature. 1974 Jun 14;249(458):647-9 PMID: 4599917
  34. The 5'-terminal nucleotide sequence of galactose messenger ribonucleic acid of Escherichia coli.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4940-4 PMID: 4612533
  35. Nucleotide sequence at the 5' extremity of turnip yellow mosaic virus genome RNA.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3168-72 PMID: 16592542
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.7.2045
Published
1979-12-11
Pages
2045-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342365
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.7.2045 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2