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PMID: 318162 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The nucleotide sequence of the 5' untranslated region of human gamma-globin mRNA.

Nucleic acids research ·Vol. 5 ·No. 10 ·1978-10-00 ·Pages 3515-22

Chang JC, Poon R, Neumann KH, Kan YW

Abstract

The nucleotide sequence of the entire 5' untranslated region of human gamma-globin mRNA has been determined. This was accomplished by analyzing complementary DNA (cRNA) synthesized from the mRNA with reverse transcriptase. The CDNA was labeled at its 3' end with 32"p using terminal deoxynucleotidyl transferase, digested with the restriction endonuclease Hae III and the end-labeled fragment isolated ans sequenced by the method of Maxam and Gilbert. Including the initiation codon AUG, the 5' untranslated region of human gamma-globin mRNA contains 57 nucleotides, compared to 41 in alpha- and 54 in beta-globin mRNA. There is very little homology between alpha and gamma sequences in the 5' region. There is considerable homology between beta- and gamma-globin mRNAs in the regions proximal and distal to the initiation codon, but the entire sequence shows less homology than the human and rabbit beta-globin mRNAs. The hexanucleotide sequence CUUCUG is found near the 5' ends of all three human globin mRNAs, suggesting a possible role of this sequence or ribosomal binding. Both guanosine and cytidine were found at the 19th nucleotide position from the 5' end of the gamma mRNA. We believe this heterogeneity arises from the difference in nucleotide sequence between the A gamma and G gamma loci.

MeSH Terms
Base Sequence Fetal Hemoglobin/genetics Globins/genetics Humans Nucleic Acid Conformation Protein Biosynthesis RNA, Messenger/genetics
Chemicals
RNA, Messenger Globins Fetal Hemoglobin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chang J C
Poon R
Neumann K H
Kan Y W
References (20)
20 references, click to expand
  1. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase.
    J Mol Biol. 1975 May 25;94(3):441-8 PMID: 1100841
  2. 'Single-stranded' DNA from phiX174 and M13 is cleaved by certain restriction endonucleases.
    Nature. 1975 Oct 2;257(5525):421-2 PMID: 809717
  3. dna, single stranded/*metab.
    Virology. 1976 Sep;73(2):561-7 PMID: 960577
  4. Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2555-8 PMID: 1058473
  5. Complete nucleotide sequence of the 5' noncoding region of human alpha-and beta-globin mRNA.
    Cell. 1977 Dec;12(4):1085-95 PMID: 597858
  6. Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):3047-51 PMID: 268654
  7. The nucleotide sequences of the untranslated 5' regions of human alpha- and beta-globin mRNAs.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):5145-9 PMID: 270752
  8. Nucleotide sequence of 3' untranslated portion of human alpha globin mRNA.
    Nucleic Acids Res. 1977 Jul;4(7):2353-68 PMID: 909779
  9. Structure-function relationship of 5' non-coding sequence of rabbit alpha- and beta-globin mRNA.
    Nature. 1977 May 19;267(5608):279-81 PMID: 865623
  10. Complete nucleotide sequence of the 5' noncoding region of rabbit beta-globin mRNA.
    Cell. 1977 Apr;10(4):549-58 PMID: 67896
  11. Human globin messenger RNA: importance of cloning for structural analysis.
    Science. 1977 Apr 8;196(4286):200-2 PMID: 847468
  12. Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.
    Cell. 1978 Mar;13(3):551-63 PMID: 77738
  13. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  14. Isolation from rat liver and sequence of a RNA fragment containing 32 nucleotides from position 5 to 36 from the 3' end of ribosomal 18S RNA.
    Nucleic Acids Res. 1978 Feb;5(2):425-34 PMID: 634793
  15. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.
    Nat New Biol. 1972 Feb 9;235(58):163-7 PMID: 4334193
  16. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  17. Full length and discrete partial reverse transcripts of globin and chorion mRNAs.
    Cell. 1975 Apr;4(4):367-78 PMID: 47272
  18. Identical 3'-terminal octanucleotide sequence in 18S ribosomal ribonucleic acid from different eukaryotes. A proposed role for this sequence in the recognition of terminator codons.
    Biochem J. 1974 Sep;141(3):609-15 PMID: 4219140
  19. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  20. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.10.3515
Published
1978-10-00
Pages
3515-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342692
Subset
IM
Databases
GENBANK
J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K02544, M18047, M19067, X00423
External Links
PubMed source DOI: 10.1093/nar/5.10.3515 PMC Full Text View journal details
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