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PMID: 270752 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The nucleotide sequences of the untranslated 5' regions of human alpha- and beta-globin mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 11 ·1977-11-00 ·Pages 5145-9

Chang JC, Temple GF, Poon R, Neumann KH, Kan YW

Abstract

The complete sequences of the untranslated 5' regions of human alpha- and beta-globin mRNAs were determined by sequence analysis of full-length cDNAs. The single-stranded cDNAs were digested with the restriction endonuclease Hae III, and the two 3'-terminal fragments of 75 and 132 nucleotides, complementary to the 5' termini of the alpha- and beta-globin mRNAs, respectively, were isolated and sequenced. Including the initiation codon AUG, the untranslated 5' regions of human alpha- and beta-globin mRNAs contain 41 and 54 nucleotides, respectively, and exhibit striking homologies with the corresponding sequences in the rabbit. Human alpha- and beta-globin mRNAs have five bases in the region of the initiation codon that may form base pairs with the 3' terminus of 18S rRNA. Stable secondary structures with hairpin loops can be constructed in the untranslated 5' regions.

MeSH Terms
Base Sequence DNA DNA Restriction Enzymes Globins/biosynthesis Humans Protein Biosynthesis RNA, Messenger
Chemicals
RNA, Messenger Globins DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chang J C
Temple G F
Poon R
Neumann K H
Kan Y W
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.11.5145
Published
1977-11-00
Pages
5145-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432117
Subset
IM
Databases
GENBANK
J00079, J00093, J00094, J00096, J00153, J00154, J00155, J00156, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179
External Links
PubMed source DOI: 10.1073/pnas.74.11.5145 PMC Full Text View journal details
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