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PMID: 325516 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential requirements for polypeptide chain initiation complex formation at the three bacteriophage R17 initiator regions.

Nucleic acids research ·Vol. 4 ·No. 1 ·1977-01-00 ·Pages 1-15

Steitz JA, Wahba AJ, Laughrea M, Moore PB

Abstract

The initiation specificity of washed E. coli ribosomes in the presence and absence of purified initiation factors and/or S1 protein has been examined in protection experiments using 32P-labelled R17 RNA. We find that the three bacteriophage initiator regions do not exhibit equal requirements for either of these components during initiation complex formation. Specifically, both factors and S1 stimulate ribosome binding to the beginnings of the coat and replicase cistrons to a greater extent than they promote recognition of the A protein initiation site. The differential effects are therefore inversely correlated with the degree of mRNA-16S rRNA complementarity exhibited by the three initiator regions. We also observe that S1 suppresses ribosome binding to spurious sites in the R17 RNA.

MeSH Terms
Bacterial Proteins/pharmacology Binding Sites Coliphages Escherichia coli/metabolism Genes Genetic Code Peptide Chain Initiation, Translational/drug effects Peptide Initiation Factors Protein Biosynthesis RNA, Viral/metabolism RNA-Dependent RNA Polymerase/metabolism Ribosomes/metabolism Viral Proteins
Chemicals
Bacterial Proteins Peptide Initiation Factors RNA, Viral Viral Proteins RNA-Dependent RNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Steitz J A
Wahba A J
Laughrea M
Moore P B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.1.1
Published
1977-01-00
Pages
1-15
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342405
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.1.1 PMC Full Text View journal details
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