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PMID: 909779 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of 3' untranslated portion of human alpha globin mRNA.

Nucleic acids research ·Vol. 4 ·No. 7 ·1977-07-00 ·Pages 2353-68

Wilson JT, deRiel JK, Forget BG, Marotta CA, Weissman SM

Abstract

We have determined the nucleotide sequence of 75 nucleotides of the 3'-untranslated portion of normal human alpha globin mRNA which corresponds to the elongated amino acid sequence of the chain termination mutant Hb Constant Spring. This was accomplished by sequence analysis of cDNA fragments obtained by restriction endonuclease or T4 endonuclease IV cleavage of human globin cDNA synthesized from globin mRNA by use of viral reverse transcriptase. Analysis of cRNA synthesized from cDNA by use of RNA polymerase provided additional confirmatory sequence information. Possible polymorphism has been identified at one site of the sequence. Our sequence overlaps with, and extends the sequence of 43 nucleotides determined by Proudfood and coworkers for the very 3'-terminal portion of human alpha globin mRNA. The complete 3'-untranslated sequence of human alpha globin mRNA (112 nucleotides including termination codon) shows little homology to that of the human or rabbit beta globin mRNAs except for the presence of the hexanucleotide sequence AAUAAA which is found in most eukaryotic mRNAs near the 3'-terminal poly (A).

MeSH Terms
Anemia, Sickle Cell/blood Avian Myeloblastosis Virus/enzymology Base Sequence DNA Restriction Enzymes DNA-Directed DNA Polymerase/metabolism Globins/biosynthesis Humans Nucleic Acid Hybridization Oligoribonucleotides/analysis Polynucleotide 5'-Hydroxyl-Kinase Protein Biosynthesis RNA, Messenger/metabolism Reticulocytes/metabolism Templates, Genetic
Chemicals
Oligoribonucleotides RNA, Messenger Globins Polynucleotide 5'-Hydroxyl-Kinase DNA-Directed DNA Polymerase DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wilson J T
deRiel J K
Forget B G
Marotta C A
Weissman S M
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.7.2353
Published
1977-07-00
Pages
2353-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342571
Subset
IM
Databases
GENBANK
J00079, J00153, J00154, J00155, J00156, K00418, X00168
External Links
PubMed source DOI: 10.1093/nar/4.7.2353 PMC Full Text View journal details
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