The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases.

Nucleic acids research ·Vol. 1 ·No. 6 ·1974-06-00 ·Pages 727-52

Subramanian KN, Pan J, Zain S, Weissman SM

Abstract

SV40 DNA is cleaved by the Eco RII and Hae restriction endonucleases to give rise to two different sets of 16 fragments each. These fragments have been ordered by analysis of the products of redigestion of one set of fragments with another restriction enzyme. The cleavage sites have been precisely mapped based on length measurements of the products of each cleavage. This provides a convenient group of small DNA fragments suitable for sequence analysis investigation of the transcripts present in infected cells, or construction of deletion substitution variants of the virus.

MeSH Terms

DNA Restriction Enzymes DNA, Viral/genetics,isolation & purification Electrophoresis, Polyacrylamide Gel Restriction Mapping Simian virus 40/chemistry,genetics

Chemicals

DNA, Viral DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Subramanian K N

Pan J

Zain S

Weissman S M

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/1.6.727

Published

1974-06-00

Pages

727-52

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343376

Subset

External Links

PubMed source DOI: 10.1093/nar/1.6.727 PMC Full Text View journal details

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The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases.

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