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PMID: 4219140 Published · ppublish English Journal Article

Identical 3'-terminal octanucleotide sequence in 18S ribosomal ribonucleic acid from different eukaryotes. A proposed role for this sequence in the recognition of terminator codons.

The Biochemical journal ·Vol. 141 ·No. 3 ·1974-09-00 ·Pages 609-15

Shine J, Dalgarno L

Abstract

The 3'-terminal sequence of 18S ribosomal RNA from Drosophila melanogaster and Saccharomyces cerevisiae was determined by stepwise degradation from the 3'-terminus and labelling with [(3)H]isoniazid. The sequence G-A-U-C-A-U-U-A(OH) was found at the 3'-terminus of both 18S rRNA species. Less extensive data for 18S RNA from a number of other eukaryotes are consistent with the same 3'-terminal sequence, and an identical sequence has previously been reported for the 3'-end of rabbit reticulocyte 18S rRNA (Hunt, 1970). These results suggest that the base sequence in this region is strongly conserved and may be identical in all eukaryotes. As the 3'-terminal hexanucleotide is complementary to eukaryotic terminator codons we discuss the possibility that the 3'-end of 18S rRNA may have a direct base-pairing role in the termination of protein synthesis.

MeSH Terms
Animals Base Sequence Cells, Cultured Chromatography, DEAE-Cellulose Cricetinae Drosophila melanogaster Electrophoresis, Paper Genetic Code Haplorhini Insecta Isoniazid Oligonucleotides/analysis Oxidation-Reduction RNA, Ribosomal/analysis Reticulocytes Saccharomyces cerevisiae
Chemicals
Oligonucleotides RNA, Ribosomal Isoniazid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shine J
Dalgarno L
References (37)
37 references, click to expand
  1. Terminal sequence studies of high-molecular-weight ribonucleic acid. The 3' termini of rabbit globin messenger ribonucleic acid.
    Biochem J. 1973 Feb;131(2):315-25 PMID: 4737318
  2. Chain termination in vitro. Studies on the specificity of amber and ochre triplets.
    Biochem Biophys Res Commun. 1968 Sep 30;32(6):1064-70 PMID: 4882878
  3. Polypeptide chain termination using synthetic polynucleotides.
    J Mol Biol. 1965 Dec;14(2):634-9 PMID: 5326419
  4. Studies of the thermal conversion of 28 S RNA of Galleria mellonella (L.) to an 18 S product.
    J Mol Biol. 1972 Feb 28;64(1):135-44 PMID: 5015395
  5. Terminal nucleotides of avian myeloblastosis virus RNA and of ribosomal RNA from chicken leukemic myeloblasts.
    Biochim Biophys Acta. 1972 Nov 9;281(4):554-63 PMID: 4347181
  6. Mammalian peptide chain termination. II. Codon specificity and GTPase activity of release factor.
    Proc Natl Acad Sci U S A. 1971 Mar;68(3):619-24 PMID: 5276771
  7. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo.
    Proc Natl Acad Sci U S A. 1971 May;68(5):964-8 PMID: 4930244
  8. Base composition and sedimentation properties of mitochondrial RNA of Saccharomyces cerebisiae.
    Biochim Biophys Acta. 1969 Jun 17;182(2):355-60 PMID: 4894014
  9. TERMINAL-SEQUENCE STUDIES OF HIGH-MOLECULAR-WEIGHT RIBONUCLEIC. THE REACTION OF PERIODATE-OXIDIZED RIBONUCLEOSIDES , 5'-RIBONUCLEOTIDES AND RIBONUCLEIC ACID WITH ISONIAZID.
    Biochem J. 1965 May;95:541-51 PMID: 14340106
  10. Ross River virus replication in cultured mosquito and mammalian cells: virus growth and correlated ultrastructural changes.
    J Gen Virol. 1973 Oct;21:109-22 PMID: 4357368
  11. Occurrence of heat-dissociable ribosomal RNA in insects: the presence of three polynucleotide chains in 26 S RNA from cultured Aedes aegypti cells.
    J Mol Biol. 1973 Mar 25;75(1):57-72 PMID: 4197338
  12. Protein synthesis.
    Annu Rev Biochem. 1973;42:397-438 PMID: 4581230
  13. The dissociation of reticulocyte polysomes into subunits and the location of messenger RNA.
    J Mol Biol. 1966 Feb;15(2):600-18 PMID: 5915184
  14. In vitro inactivation of ascites ribosomes by colicin E 3.
    Biochem Biophys Res Commun. 1973 May 1;52(1):327-34 PMID: 4736471
  15. Conserved terminal sequence in 18SrRNA may represent terminator anticodons.
    Nat New Biol. 1973 Oct 31;245(148):261-2 PMID: 4202225
  16. The effect of antibiotics on lipid synthesis during respiratory development in Saccharomyces cerevisiae.
    Microbios. 1971 Sep;4(14):115-32 PMID: 4949964
  17. Characterization of three proteins involved in polypeptide chain termination.
    Cold Spring Harb Symp Quant Biol. 1969;34:469-77 PMID: 4909514
  18. Demonstration of UAG as a nonsense codon in bakers' yeast by amino-acid replacements in iso-1-cytochrome c.
    J Mol Biol. 1972 Jul 28;68(3):429-43 PMID: 4560848
  19. Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1971 May;68(5):959-63 PMID: 4930243
  20. Identification of nonsense codons in yeast.
    J Mol Biol. 1969 Jul 14;43(1):71-5 PMID: 5811824
  21. Partial nucleotide sequence of 16S ribosomal RNA from E. coli.
    Nat New Biol. 1972 Sep 6;239(88):1-5 PMID: 4562230
  22. Initiation of eukaryotic protein synthesis: (Met-tRNA f -40S ribosome) initiation complex catalysed by purified initiation factors in the absence of mRNA.
    Nat New Biol. 1973 Mar 14;242(115):35-8 PMID: 4512006
  23. Polypeptide chain termination in vitro: isolation of a release factor.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):1144-51 PMID: 5233840
  24. Studies on the 3'-terminal sequences of the large ribosomal ribonucleic acid of different eukaryotes and those associated with "hidden" breaks in heart-dissociable insects 26S ribonucleic acid.
    Biochem J. 1974 Sep;141(3):617-25 PMID: 4219141
  25. Codon--anticodon pairing: the wobble hypothesis.
    J Mol Biol. 1966 Aug;19(2):548-55 PMID: 5969078
  26. Interaction of colicins with bacterial cells. II. Specific alteration of Escherichia coli ribosomes induced by colicin E3 in vivo.
    J Mol Biol. 1967 Jun 14;26(2):181-95 PMID: 5340611
  27. Establishment of four strains of cells from insect tissues grown in vitro.
    Nature. 1962 Aug 25;195:788-9 PMID: 13900940
  28. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  29. Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli.
    Nat New Biol. 1971 Sep 1;233(35):12-4 PMID: 4329247
  30. Kasugamycin resistance: 30S ribosomal mutation with an unusual location on the Escherichia coli chromosome.
    Science. 1970 Jan 2;167(3914):56-8 PMID: 4901406
  31. Secondary methylation of yeast ribosomal RNA.
    FEBS Lett. 1972 Oct 15;27(1):102-106 PMID: 11946817
  32. Release factors differing in specificity for terminator codons.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):768-74 PMID: 4879404
  33. The nucleic acids of Drosophila melanogaster.
    Biochem J. 1966 Aug;100(2):532-9 PMID: 5968550
  34. Terminal-sequence studies of high-molecular-weight ribonucleic acid. The 3'-termini of rabbit reticulocyte ribosomal RNA.
    Biochem J. 1970 Nov;120(2):353-63 PMID: 4321896
  35. Polypeptide chain termination: an active process.
    J Mol Biol. 1968 May 28;34(1):131-6 PMID: 4938538
  36. Early and late methylations in HeLa cell ribosome maturation.
    Nature. 1973 Aug 10;244(5415):334-6 PMID: 4583505
  37. Peptide chain termination. V. The role of release factors in mRNA terminator codon recognition.
    Proc Natl Acad Sci U S A. 1969 Dec;64(4):1235-41 PMID: 4916922
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1410609a
Published
1974-09-00
Pages
609-15
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168164
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1410609a PMC Full Text View journal details
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