Initiation of eukaryotic protein synthesis: (Met-tRNA f -40S ribosome) initiation complex catalysed by purified initiation factors in the absence of mRNA.

Animals Carbon Isotopes Carcinoma, Krebs 2 Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Globins/biosynthesis Guanosine Triphosphate/metabolism Guinea Pigs Leucine/metabolism Liver/analysis Methionine Mice Models, Biological Peptide Chain Initiation, Translational Peptide Initiation Factors/isolation & purification Protein Binding RNA, Messenger/metabolism RNA, Transfer/metabolism Rabbits Reticulocytes/analysis Ribosomes/metabolism Templates, Genetic Tritium

Carbon Isotopes Peptide Initiation Factors RNA, Messenger Tritium Guanosine Triphosphate Globins RNA, Transfer Methionine Leucine