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PMID: 4930243 Published · ppublish English Journal Article

Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 5 ·1971-05-00 ·Pages 959-63

Senior BW, Holland IB

Abstract

The properties of 30S ribosomal subunits from untreated and from colicin E3-treated (E3-30S) bacteria have been compared. Polyacrylamide gel electrophoresis of ribosomal proteins revealed no difference, but several studies indicated that the 16S RNA from E3-30S particles was modified. E3-16S RNA showed slightly increased resistance to heat-induced degradation and had a reduced (15 S) sedimentation coefficient on sucrose gradients. Fingerprint analyses of E3-16S RNA revealed that the 3'-terminus of the molecule had been deleted. It was concluded that a primary effect of colicin E3 is the activation of a highly specific RNase that degrades 30S ribosomal RNA in situ, and that the resulting fragment(s) are probably retained within the 30S particle.

MeSH Terms
Autoradiography Bacterial Proteins/analysis Centrifugation, Density Gradient Colicins/pharmacology Densitometry Electrophoresis, Disc Escherichia coli/cytology Nucleoproteins/analysis RNA, Ribosomal/metabolism Ribonucleases/metabolism Ribosomes/analysis,drug effects
Chemicals
Bacterial Proteins Colicins Nucleoproteins RNA, Ribosomal Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Senior B W
Holland I B
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.5.959
Published
1971-05-00
Pages
959-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389090
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.5.959 PMC Full Text View journal details
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