Home LiteratureArticle Details
PMID: 4898995 Published · ppublish English Journal Article

Kinetics of adsorption of colicin CA42-E2 and reversal of its bactericidal activity.

Journal of bacteriology ·Vol. 100 ·No. 1 ·1969-10-00 ·Pages 301-9

Reynolds BL, Reeves PR

Abstract

The kinetics of killing of Escherichia coli K-12 by colicin CA42-E2 have been studied, and the data were used to estimate the adsorption constant of this colicin under various environmental conditions. Evidence was obtained suggesting that the adsorption of colicin occurred in two stages; the earlier stage was reversible and did not lead to the death of the cell, the latter stage was irreversible and bactericidal. Cells which had adsorbed a lethal quantity of colicin could be rescued for a short time by inactivating the adsorbed colicin with trypsin. However, when the metabolic activity of the cells was totally arrested the lethal effect of adsorbed colicin was subject to trypsin reversal over long periods of time.

MeSH Terms
Adsorption Colicins/antagonists & inhibitors,pharmacology Escherichia coli/drug effects,enzymology,growth & development Kinetics Magnesium/pharmacology Time Factors Trypsin/metabolism Zinc/pharmacology
Chemicals
Colicins Trypsin Magnesium Zinc
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reynolds B L
Reeves P R
References (19)
19 references, click to expand
  1. The action of T2 bacteriophage ghosts on Escherichia coli B.
    Can J Microbiol. 1955 Dec;1(9):757-74 PMID: 13270153
  2. Mutants of Escherichia coli requiring methionine or vitamin B12.
    J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
  3. The mechanism of virus attachment to host cells. I. The role of ions in the primary reaction.
    J Exp Med. 1951 Jan;93(1):65-88 PMID: 14803632
  4. The first two steps of the invasion of host cells by bacterial viruses. II.
    J Exp Med. 1951 Sep;94(3):177-89 PMID: 14861377
  5. THE ADSORPTION AND KINETICS OF KILLING BY COLICIN CA42-E2.
    Aust J Exp Biol Med Sci. 1965 Apr;43:191-200 PMID: 14323559
  6. Interaction of colicins with bacterial cells. I. Studies with radioactive colicins.
    J Bacteriol. 1966 Feb;91(2):685-94 PMID: 5327363
  7. The mechanism of virus attachment to host cells. IV. Physicochemical studies on virus and cell surface groups.
    Arch Biochem Biophys. 1954 Jul;51(1):229-45 PMID: 13181477
  8. Kinetics of lethal adsorption of colicin E-2 by Escherichia coli.
    J Bacteriol. 1967 Apr;93(4):1353-9 PMID: 5340307
  9. Improved method for obtaining thymineless mutants of Escherichia coli and Salmonella typhimurium.
    J Bacteriol. 1962 Sep;84:602-3 PMID: 13939760
  10. [Bactericidal action of colicine K].
    C R Seances Soc Biol Fil. 1952 Aug;146(15-16):1295-7 PMID: 13020144
  11. An examination of single-hit and multi-hit hypotheses in relation to the possible kinetics of colicin adsorption.
    J Theor Biol. 1966 Aug;11(3):383-410 PMID: 5967439
  12. Protection of Escherichia coli from the lethal effect of colicins by high osmotic pressure.
    J Bacteriol. 1967 Jan;93(1):80-5 PMID: 5335904
  13. Thermodynamic and kinetic studies on the attachment of T1 bacteriophage to bacteria.
    Biochim Biophys Acta. 1954 Jun;14(2):163-72 PMID: 13172233
  14. Colicine K. I. The production of colicine K in media maintained at constant pH.
    J Exp Med. 1956 May 1;103(5):577-88 PMID: 13319579
  15. Gene Recombination in the Bacterium Escherichia coli.
    J Bacteriol. 1947 Jun;53(6):673-84 PMID: 16561324
  16. [Bacteriologic analysis of the colicine complexes produced by 14 antibiotic strains of Escherichia coli].
    Ann Inst Pasteur (Paris). 1950 Jul;79(1):51-9 PMID: 14771606
  17. [Biosynthesis of a colicin and its mode of action].
    Ann Inst Pasteur (Paris). 1952 Sep;83(3):295-315 PMID: 13017127
  18. Reversibility of inhibition of nucleic acids and protein synthesis by colicin K.
    Biochem Biophys Res Commun. 1962 May 4;7:306-9 PMID: 14480262
  19. The protein coats or ghosts of coli phage T2. II. The biological functions.
    J Gen Physiol. 1957 Nov 20;41(2):307-31 PMID: 13475694
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1969-10-00
Pages
301-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC315393
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com