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PMID: 5276771 Published · ppublish English Journal Article

Mammalian peptide chain termination. II. Codon specificity and GTPase activity of release factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 3 ·1971-03-00 ·Pages 619-24

Beaudet AL, Caskey CT

Abstract

In vitro peptide chain termination with release factor preparations from rabbit reticulocytes, guinea pig liver, or Chinese hamster liver is directed with UAAA, UAGA, or UGAA, suggesting that UAA, UAG, and UGA are terminator condons for mammalian cells. Purified release factor from rabbit reticulocytes has ribosomaldependent GTPase activity, which is stimulated by UAAA. GTP hydrolysis appears requisite for in vitro peptide chain termination in mammals.

MeSH Terms
Animals Bacterial Proteins/biosynthesis Blood Proteins/biosynthesis
Chemicals
Bacterial Proteins Blood Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Beaudet A L
Caskey C T
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.3.619
Published
1971-03-00
Pages
619-24
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389002
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.3.619 PMC Full Text View journal details
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