Peptide chain termination: effect of protein S on ribosomal binding of release factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 2 ·1970-10-00 ·Pages 537-43

Goldstein JL, Caskey CT

Abstract

THE PROTEIN FACTOR S, PREVIOUSLY SHOWN TO STIMULATE POLYPEPTIDE CHAIN TERMINATION IN BACTERIAL EXTRACTS, HAS TWO EFFECTS UPON THE COMPLEX FORMED BETWEEN RIBOSOMES, RELEASE FACTOR, AND TERMINATOR (TRINUCLEOTIDE) CODON: (1) in the absence of GTP or GDP, S stimulates formation of an [R.UAA.ribosome] intermediate, and (2) in the presence of GTP or GDP, S participates in dissociation of this intermediate. Factor S can stimulate fMet release from [fMet-tRNA(f).AUG.ribosome] intermediates in either the presence or absence of GTP or GDP. A model is proposed which relates the in vitro effects of S +/- GTP (or GDP) on fMet release to the effects of S +/- GTP (or GDP) on the binding and dissociation of R factor from ribosomes.

MeSH Terms

Base Sequence Formates/metabolism Guanine Nucleotides/metabolism Guanosine Triphosphate/metabolism Methionine/metabolism Peptide Biosynthesis Peptide Chain Termination, Translational Polynucleotides Protein Binding Proteins/metabolism RNA, Transfer/metabolism Ribosomes/metabolism Tritium

Chemicals

Formates Guanine Nucleotides Polynucleotides Proteins Tritium Guanosine Triphosphate RNA, Transfer Methionine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Goldstein J L

Caskey C T

References (11)

11 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.2.537

Published

1970-10-00

Pages

537-43

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283241

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.2.537 PMC Full Text View journal details

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Peptide chain termination: effect of protein S on ribosomal binding of release factors.

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