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PMID: 4905675 Published · ppublish English Journal Article

Peptide chain termination. VI. Purification and site of action of S.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 2 ·1970-02-00 ·Pages 430-7

Goldstein J, Milman G, Scolnick E, Caskey T

Abstract

PEPTIDE CHAIN TERMINATION IS A RESULT OF AT LEAST TWO EVENTS: terminator codon recognition and hydrolysis of peptidyl tRNA. A protein factor S, isolated from the supernatant of Escherichia coli B, stimulates fMet release. Factor S lowers the K(m) for terminator trinucleotides without altering the V(max) of release and therefore acts at terminator codon recognition. The S protein differs from initiation factors, elongation factor G, several forms of elongation factor T and release factors. The importance of the 2% Tu content in purified S is unresolved.

MeSH Terms
Bacterial Proteins/isolation & purification,metabolism Escherichia coli/metabolism Formates/metabolism Genetic Code Kinetics Methionine Nucleotides/metabolism Peptide Biosynthesis RNA, Transfer/metabolism Uracil Nucleotides
Chemicals
Bacterial Proteins Formates Nucleotides Uracil Nucleotides RNA, Transfer Methionine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Goldstein J
Milman G
Scolnick E
Caskey T
References (11)
11 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.65.2.430
Published
1970-02-00
Pages
430-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282921
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.65.2.430 PMC Full Text View journal details
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