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PMID: 4917818 Published · ppublish English Journal Article

Peptide chain termination with mammalian release factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 1 ·1970-09-00 ·Pages 99-106

Goldstein JL, Beaudet AL, Caskey CT

Abstract

We report a method for the in vitro study of peptide chain termination in mammals. A proteinaceous release factor has been isolated from rabbit reticulocyte extracts. This factor and a polyribonucleotide template containing the bases U and A stimulate the release of N-formylmethionine from mammalian [N-formylmethionyl-tRNA(f).ribosome] intermediates. Our studies suggest that UAA is a terminator codon for mammalian cells.

MeSH Terms
Animals Anti-Bacterial Agents/pharmacology Escherichia coli Genetic Code In Vitro Techniques Methionine/metabolism Peptide Biosynthesis Polynucleotides Proteins/isolation & purification RNA, Transfer Rabbits Reticulocytes Ribosomes Templates, Genetic Tritium
Chemicals
Anti-Bacterial Agents Polynucleotides Proteins Tritium RNA, Transfer Methionine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goldstein J L
Beaudet A L
Caskey C T
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.67.1.99
Published
1970-09-00
Pages
99-106
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC283173
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.67.1.99 PMC Full Text View journal details
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