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PMID: 4737318 Published · ppublish English Journal Article

Terminal sequence studies of high-molecular-weight ribonucleic acid. The 3' termini of rabbit globin messenger ribonucleic acid.

The Biochemical journal ·Vol. 131 ·No. 2 ·1973-02-00 ·Pages 315-25

Hunt JA

Abstract

Haemoglobin mRNA isolated from EDTA-treated polyribosomes has an apparent molecular weight of 120000-180000 estimated by condensation with (3)H-labelled isoniazid after periodate oxidation. Analysis of the ribonuclease digests of isoniazid-labelled RNA by paper electrophoresis and column chromatography enables the amount of contaminating 18S, 7S, 5S and 4S RNA to be estimated, and a corrected molecular weight of globin mRNA as the acid is 161000 or 500 nucleotides in length. This molecule contains two groups of 3'-terminal sequences in equal yield; G-Y-A(6) and G-Y-A(7) in the ratio 3:2, and G-N(9-16)-Y-A(2) and G-N(9-16)-Y-N(3) in the ratio 3:2. The significance of these sequences is discussed in relation to the poly(A) content of globin mRNA, the specificity of the sequences, and possible function in processing and biosynthesis of mRNA.

MeSH Terms
Animals Base Sequence Binding Sites Carbon Isotopes Centrifugation, Density Gradient Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Electrophoresis, Paper Electrophoresis, Polyacrylamide Gel Globins Isoniazid Molecular Weight Oligonucleotides/analysis Pancreas RNA, Messenger/analysis,isolation & purification RNA, Ribosomal/analysis,isolation & purification Rabbits Reticulocytes/analysis,cytology Ribonucleases Ribonucleotides/analysis Spectrophotometry Spectrophotometry, Ultraviolet Tritium
Chemicals
Carbon Isotopes Oligonucleotides RNA, Messenger RNA, Ribosomal Ribonucleotides Tritium Globins Ribonucleases Isoniazid
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hunt J A
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1310315
Published
1973-02-00
Pages
315-25
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1177471
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1310315 PMC Full Text View journal details
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