Interaction between polyuridylic acid and rabbit globin messenger ribonucleic acid.

The Biochemical journal ·Vol. 131 ·No. 2 ·1973-02-00 ·Pages 327-33

Hunt JA

Abstract

Poly(U) binds to globin mRNA in 0.1m-NaCl. Studies with ribonuclease digestion of this complex suggest that there are polyadenylate-rich sequences in the mRNA containing about 30-40 adenylate residues. The sequences appear to be homogenous and of approximately the same length for both alpha- and beta-globin mRNA. They are most likely located at the 3' terminus of the molecule.

MeSH Terms

Animals Binding Sites Carbon Isotopes Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Globins Nucleic Acid Hybridization Oligonucleotides/analysis Pancreas Phosphoric Diester Hydrolases Poly U Polynucleotides RNA, Messenger Rats Reticulocytes Ribonucleases Snakes Spectrophotometry, Ultraviolet Spleen Temperature Time Factors Tritium Venoms

Chemicals

Carbon Isotopes Oligonucleotides Polynucleotides RNA, Messenger Venoms Tritium Poly U Globins Ribonucleases Phosphoric Diester Hydrolases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Hunt J A

References (8)

8 references, click to expand

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J Mol Biol. 1966 Aug;19(1):145-60 PMID: 5967281
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
Terminal sequence studies of high-molecular-weight ribonucleic acid. The 3' termini of rabbit globin messenger ribonucleic acid.
Biochem J. 1973 Feb;131(2):315-25 PMID: 4737318
[Thermodynamics of poly A and poly U complexes in neutral and acid media].
Eur J Biochem. 1968 Feb;3(4):428-38 PMID: 5642453
Adenine-rich polymer associated with rabbit reticulocyte messenger RNA.
Nature. 1970 Aug 15;227(5259):710-2 PMID: 5432070
Poly A sequences at the 3' termini of rabbit globin mRNAs.
Nat New Biol. 1971 Sep 8;233(36):41-3 PMID: 5286353
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1310327

Published

1973-02-00

Pages

327-33

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177472

Subset

External Links

PubMed source DOI: 10.1042/bj1310327 PMC Full Text View journal details

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Interaction between polyuridylic acid and rabbit globin messenger ribonucleic acid.

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