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PMID: 673854 Published · ppublish English Journal Article

Nucleotide sequence at the 5' end of ovalbumin messenger RNA from chicken.

Nucleic acids research ·Vol. 5 ·No. 7 ·1978-07-00 ·Pages 2253-66

Kuebbing D, Liarakos CD

Abstract

DNA-sequence analysis of 300 nucleotides from the region of cloned, double-stranded ovalbumin cDNA corresponding to the 5' end of ovalbumin messenger RNA was accomplished using the technique of Maxam and Gilbert (Proc. Nat. Acad. Sci. USA (1977) 74,560-564). The AUG initiation codon was located 52 nucleotides from the AT linkers used in cloning and immediately adjacent to the amino terminal peptide of ovalbumin, indicating the absence of a "signal peptide" in this protein. The nucleotide sequence coding for a phosphorylated peptide from ovalbumin was also found. These results demonstrate that the coding portion of mRNAov begins near the 5' end of the molecule leaving some 600 nucleotides of noncoding information at the 3' end.

MeSH Terms
Animals Base Sequence Chickens Codon DNA, Recombinant Female Hydrogen Bonding Nucleic Acid Conformation Ovalbumin/biosynthesis,genetics Peptide Chain Initiation, Translational Protein Precursors/biosynthesis RNA, Messenger/genetics
Chemicals
Codon DNA, Recombinant Protein Precursors RNA, Messenger Ovalbumin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kuebbing D
Liarakos C D
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.7.2253
Published
1978-07-00
Pages
2253-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342161
Subset
IM
Databases
GENBANK
V00383
External Links
PubMed source DOI: 10.1093/nar/5.7.2253 PMC Full Text View journal details
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