Home LiteratureArticle Details
PMID: 400797 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Area of 16S ribonucleic acid at or near the interface between 30S and 50S ribosomes of Escherichia coli.

Journal of bacteriology ·Vol. 130 ·No. 2 ·1977-05-00 ·Pages 900-10

Santer M, Shane S

Abstract

To determine the region of 16S ribonucleic acid (RNA) at the interface between 30 and 50S ribosomes of Escherichia coli, 30 and 70S ribosomes were treated with T1 ribonuclease (RNase). The accessibility of 16S RNA in the 5' half of the molecule is the same in 30 and 70S ribosomes. The interaction with 50S ribosomes decreases the sensitivity to T1 RNase of an area in the middle of 16S RNA. A large area near the 3' end of 16S RNA is completely protected in 70S ribosomes. The RNA near the 3' end of the molecule and an area of RNA in the middle of the molecule appear to be at the interface between 30 and 50S ribosomes. One site in 16S RNA, 13 to 15 nucleotides from the 3' end, normally inaccessible to T1 RNase in 30S ribosomes, becomes accessible to T1 RNase in 70S ribosomes. This indicates a conformational change at the 3' end of 16S RNA when 30S ribosomes are associated with 50S ribosomes.

MeSH Terms
Ammonium Chloride/pharmacology Base Sequence Escherichia coli/analysis Molecular Weight Nucleic Acid Conformation Oligonucleotides/analysis RNA, Ribosomal/analysis Ribonucleases/pharmacology Ribosomes/analysis
Chemicals
Oligonucleotides RNA, Ribosomal Ammonium Chloride Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Santer M
Shane S
References (30)
30 references, click to expand
  1. Ribosome structure determined by electron microscopy of Escherichia coli small subunits, large subunits and monomeric ribosomes.
    J Mol Biol. 1976 Jul 25;105(1):131-9 PMID: 792456
  2. Nucleotide sequence from the coat protein cistron of R17 bacteriophage RNA.
    Nature. 1969 Sep 6;223(5210):1009-14 PMID: 5811898
  3. The immunological reactivity of 30s ribosomal proteins in 70s ribosomes from Escherichia coli.
    Biochem Biophys Res Commun. 1973 Nov 1;55(1):162-8 PMID: 4131533
  4. Topography of 16S RNA in 30S ribosomal subunits. Nucleotide sequences and location of sites of reaction with kethoxal.
    Biochemistry. 1974 Nov 5;13(23):4694-703 PMID: 4371816
  5. Action of ribonuclease T1 on 30S ribosomes of Escherichia coli and its role in sequence studies on 16S ribonucleic acid.
    J Bacteriol. 1973 Dec;116(3):1304-13 PMID: 4356617
  6. Protein-protein proximity in the association of ribosomal subunits of Escherichia coli: crosslinking of 30 S protein S16 to 50 S proteins by glutaraldehyde or formaldehyde.
    J Mol Biol. 1974 Aug 15;87(3):509-22 PMID: 4216639
  7. Nucleotide sequence of the 3' terminus of E. coli 16S ribosomal RNA.
    Mol Biol Rep. 1974 Dec;1(8):437-9 PMID: 4616170
  8. Ribosomal protein neighborhoods. I. S18 and S21 as well as S5 and S8 are neighbors.
    Mol Gen Genet. 1972;119(4):357-66 PMID: 4630861
  9. Letter: Conformational changes in ribosomal subunits following dissociation of the Escherichia coli 70 S ribosome.
    J Mol Biol. 1973 Aug 15;78(3):563-8 PMID: 4585330
  10. Topography of the Escherichia coli 30 S ribosome revealed by the modification of ribosomal proteins.
    J Mol Biol. 1973 Aug 15;78(3):539-50 PMID: 4585328
  11. Coordinate inhibition of elongation factor G function and ribosomal subunit association by antibodies to several ribosomal proteins.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):627-30 PMID: 4595567
  12. Proteins occurring at, or near, the subunit interface of E. coli ribosomes.
    Mol Gen Genet. 1973 Dec 31;127(4):359-68 PMID: 4594008
  13. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  14. Characterization of the 5' and 3' ends of the 16S ribonucleic acid from T 1 -ribonuclease treated 30S ribosomes.
    Biochemistry. 1972 Feb 29;11(5):786-91 PMID: 4551094
  15. Surface topography of the 30 s Escherichia coli ribosomal subunit: reactivity towards fluorescein isothiocyanate.
    J Mol Biol. 1972 Jun 20;67(2):265-75 PMID: 4557203
  16. The sequence of 5 s ribosomal ribonucleic acid.
    J Mol Biol. 1968 Jun 28;34(3):379-412 PMID: 4938553
  17. Resistance of E. coli ribosomal 5S RNA to degradation by ribonuclease in reconstituted ribosomal particles.
    Biochem Biophys Res Commun. 1970 Apr 8;39(1):114-22 PMID: 4908845
  18. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo.
    Proc Natl Acad Sci U S A. 1971 May;68(5):964-8 PMID: 4930244
  19. Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1971 May;68(5):959-63 PMID: 4930243
  20. A two-dimensional fractionation procedure for radioactive nucleotides.
    J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
  21. Nucleotide sequences of the T1 and pancreatic ribonuclease digestion products from some large fragments of the 23S RNA of Escherichia coli.
    Biochimie. 1975;57(2):175-225 PMID: 806306
  22. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  23. Conservation of primary structure in 16S ribosomal RNA.
    Nature. 1975 Mar 6;254(5495):83-6 PMID: 1089909
  24. Characterization of fragments of 16 S ribonucleic acid protected against pancreatic ribonuclease digestion by ribosomal protein S4.
    J Biol Chem. 1975 Jun 10;250(11):4100-12 PMID: 1092684
  25. Primary sequence of the 16S ribosomal RNA of Escherichia coli.
    Nucleic Acids Res. 1975 Feb;2(2):265-78 PMID: 1091918
  26. Studies of 30 S Escherichia coli ribosome reassembly using individual proteins labeled with an environmentally sensitive fluorescent prode.
    J Mol Biol. 1975 Oct 5;97(4):423-41 PMID: 1102711
  27. The role of 16S rRNA in ribosomal binding of IF-3.
    Biochemistry. 1976 Feb 24;15(4):804-11 PMID: 764867
  28. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8 PMID: 1107998
  29. New aspects of the IF3-ribosome interaction.
    FEBS Lett. 1976 Feb 15;62(2):111-4 PMID: 767141
  30. The use of ribonuclease U2 in RNA sequence determination. Some corrections in the catalog of oligomers produced by ribonuclease T1 digestion of Escherichia coli 16S ribosomal RNA.
    J Mol Evol. 1974 Feb 28;3(1):63-77 PMID: 4597768
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.130.2.900-910.1977
Published
1977-05-00
Pages
900-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235296
Subset
IM
Grants
NIGMS NIH HHS · GM 11738 · United States
External Links
PubMed source DOI: 10.1128/jb.130.2.900-910.1977 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2