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PMID: 6258158 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence through the 18S-28S intergene region of a vertebrate ribosomal transcription unit.

Nucleic acids research ·Vol. 8 ·No. 24 ·1980-12-20 ·Pages 5993-6005

Hall LM, Maden BE

Abstract

We have determined the nucleotide sequence of part of a cloned ribosomal transcription unit from Xenopus laevis extending from the 3' region of the 18S gene through the 18S-28S intergene region into the start of the 28S gene. The 18S 3' region possess two tracts of high homology with the corresponding segments of other eukaryotic 18S genes (yeast and Bombyx mori) separated by a tract of low homology which in X. laevis is rich in G plus C. The first internal transcribed spacer, between the 18S and 5.8S genes, is 557 nucleotides long, very rich in G plus C (84%) and shows no sequence homology with the corresponding yeast sequence. The 5.8S rRNA sequence is revised slightly in the light of the DNA sequence. The second internal transcribed spacer, between the 5.8S and 28S genes, is 262 nucleotides long and is even richer in G plus C (88%) than the first internal spacer. 28S rRNA starts with the sequence pUCAG. This is encoded at the first of three closely linked TCAG sites in rDNA.

MeSH Terms

Animals Base Sequence Bombyx/genetics Cloning, Molecular DNA DNA Restriction Enzymes Nucleic Acid Hybridization RNA, Ribosomal Ribosomes Saccharomyces cerevisiae/genetics Transcription, Genetic Xenopus laevis/genetics

Chemicals

RNA, Ribosomal DNA DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hall L M

Maden B E

References (27)

27 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.24.5993

Published

1980-12-20

Pages

5993-6005

Language

English

Region

England

NLM ID

0411011

PMCID

PMC328067

Subset

Databases

GENBANK

J00999

External Links

PubMed source DOI: 10.1093/nar/8.24.5993 PMC Full Text View journal details

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Nucleotide sequence through the 18S-28S intergene region of a vertebrate ribosomal transcription unit.

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