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PMID: 388355 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fine structure of ribosomal RNA. II. Distribution of methylated sequences within Xenopus laevis rRNA.

Nucleic acids research ·Vol. 7 ·No. 6 ·1979-11-24 ·Pages 1497-511

Brand RC, Gerbi SA

Abstract

The distribution of methyl groups in rRNA from Xenopus laevis was analyzed by hybridization of rRNA to subfragments of either of two cloned rDNA fragments, X1r11 and X1r12, which together constitute a complete rDNA repeat unit. Using a mixture of 3H-methyl plus 32P-labelled rRNA as probe, the molar yield of methyl groups per rRNA region in hybrid could be calculated. For this calculation the length of the rRNA coding region in each DNA subfragment is needed, which was determined for X1r11 subfragments by the nuclease S1 mapping method of Berk and Sharp. The results show that both in 18S and 28S rRNA the methyl groups are nonrandomly distributed. For 18S rRNA, clustering was found within a 3' terminal fragment of 310 nucleotides. For 28S rRNA, clustering of methyl groups was found within a region of 750 nucleotides in length, which ends 500 nucleotides from the 3' end. In contrast, the 28S rRNA 5' terminal region of 900 nucleotides is clearly undermethylated. The general position of methyl groups in 28S rRNA correlates with the location of evolutionarily conserved sequences in this molecule, as recently determined in our laboratory.

MeSH Terms
Animals Cells, Cultured DNA Restriction Enzymes DNA, Recombinant Escherichia coli/metabolism Kidney Kinetics Methylation Nucleic Acid Hybridization Plasmids RNA, Ribosomal Xenopus
Chemicals
DNA, Recombinant RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brand R C
Gerbi S A
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44 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.6.1497
Published
1979-11-24
Pages
1497-511
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342323
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.6.1497 PMC Full Text View journal details
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