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PMID: 6253895 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence encoding the 5' end of Xenopus laevis 18S rRNA.

Nucleic acids research ·Vol. 8 ·No. 13 ·1980-07-11 ·Pages 2871-84

Salim M, Maden BE

Abstract

We have sequenced a region of cloned Xenopus laevis ribosomal DNA encompassing the last 24 nucleotides of the external transcribed spacer and the first 275 nucleotides of the 18S gene. The start of the 18S gene was identified by correlating the results obtained from RNA hybridization and fingerprinting with the DNA sequence. This 5' region of 18S rRNA contains five 2'-O-methyl groups and at least six pseudouridine residues. Several of these modified nucleotides are clustered into a relatively short region from nucleotides 99-124. Nucleotides 227-250 constitute a distinctive sequence of 24 consecutive G and C residues. Comparison with the first 160 nucleotides of a yeast 18S gene (25) reveals three blocks of high sequence homology separated by two short tracts where homology is low or absent. The external transcribed spacer sequences diverge widely from within a few nucleotides of the start of the 18S gene.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Molecular Weight Nucleic Acid Hybridization RNA, Ribosomal/biosynthesis Transcription, Genetic Xenopus
Chemicals
DNA, Recombinant RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Salim M
Maden B E
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.13.2871
Published
1980-07-11
Pages
2871-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324131
Subset
IM
Databases
GENBANK
J00999
External Links
PubMed source DOI: 10.1093/nar/8.13.2871 PMC Full Text View journal details
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