Laser Raman evidence for new cloverleaf secondary structures for eukaryotic 5.8S RNA and prokaryotic 5S RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4901-5

Luoma GA, Marshall AG

Abstract

Neither of the two previously proposed secondary structures for eukaryotic 5.8S RNA is consistent with the present laser Raman results. A new, highly stable "cloverleaf" secondary structure not only fits the Raman data but also accounts for previously determined enzymatic partial cleavage patterns, base sequence and pairing homologies, and G-C and A-U base pair numbers and ratios. The new cloverleaf model also conserves several structural features (constant loops, bulges, and stems) consistent with known 5.8S RNA functions. Finally, we propose a similar new cloverleaf secondary structure for Escherichia coli 5S RNA, consonant with many known properties of prokaryotic 5S RNA.

MeSH Terms

Chemical Phenomena Chemistry Escherichia coli/genetics Eukaryotic Cells Models, Molecular Models, Structural Prokaryotic Cells RNA Spectrum Analysis, Raman

Chemicals

RNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Luoma G A

Marshall A G

References (29)

29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.10.4901

Published

1978-10-00

Pages

4901-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC336229

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.10.4901 PMC Full Text View journal details

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Laser Raman evidence for new cloverleaf secondary structures for eukaryotic 5.8S RNA and prokaryotic 5S RNA.

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