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PMID: 6153795 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 1 ·1980-01-00 ·Pages 201-4

Brosius J, Dull TJ, Noller HF

Abstract

The complete nucleotide sequence of the 23S RNA gene from the rrnB operon of Escherichia coli has been determined. The sequences of both strands of the entire gene were determined, most of the sequence was independently confirmed by use of alternate restriction fragments, and all restriction cuts overlapped. The DNA region corresponding to mature 23S rRNA contains 2904 nucleotides. Kethoxal-reactive sites protected by 30S subunits are found between positions 2300 and 28000, placing the subunit interface in this region of the molecule. The functional importance of this region is further supported by studies by other investigators, including homology with chloroplast and mitochondrial rRNA.

MeSH Terms
Base Sequence Chloroplasts Escherichia coli/genetics Genes Mitochondria Molecular Weight RNA, Bacterial/genetics RNA, Ribosomal/genetics RNA, Transfer/genetics
Chemicals
RNA, Bacterial RNA, Ribosomal RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brosius J
Dull T J
Noller H F
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.1.201
Published
1980-01-00
Pages
201-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC348236
Subset
IM
Databases
GENBANK
J01695
External Links
PubMed source DOI: 10.1073/pnas.77.1.201 PMC Full Text View journal details
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