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PMID: 370791 Published · ppublish English Journal Article

Sequence of the gene for isoleucine tRNA1 and the surrounding region in a ribosomal RNA operon of Escherichia coli.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 575-92

Sekiya T, Nishimura S

Abstract

A DNA fragment of about 2000 base pairs carrying the gene for tRNA(1) (Ile) has been cloned from a total Eco RI endonuclease digest of Escherichia coli DNA. Sequence analyses revealed that about the first 850 base pairs from one end of the fragment contain a nucleotide sequence corresponding to that in the 3'-end of 16S rRNA. The gene for tRNA(Ile) follows the 16S rRNA gene and both genes flank a spacer sequence of 68 base pairs. The spacer region contains a repeating, a hair pin and a symmetrical structure when the sequence is viewed in the single stranded form. A notable hair pin structure is also observed in the region adjacent to the 3'-end of the tRNA(1) (Ile) gene. In addition, about 850 base pairs from the other end of the DNA fragment have been found to contain the nucleotide sequence of the 5'-end of 23S rRNA. The presence of the genes for tRNA(1) (Ile), 16S and 23S rRNA and the hybridization to tRNA(1) (Ala) suggest that this cloned DNA is part of one of the E. coli rRNA operons carrying these two tRNA genes as a spacer.Images

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Bacterial/metabolism Escherichia coli/metabolism Isoleucine Molecular Weight Operon RNA, Ribosomal/biosynthesis RNA, Transfer/biosynthesis
Chemicals
DNA, Bacterial RNA, Ribosomal Isoleucine RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sekiya T
Nishimura S
References (34)
34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.2.575
Published
1979-02-00
Pages
575-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327714
Subset
IM
Databases
GENBANK
K01056, K01057, K01058
External Links
PubMed source DOI: 10.1093/nar/6.2.575 PMC Full Text View journal details
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