Home LiteratureArticle Details
PMID: 345126 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A rho-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli.

Nature ·Vol. 272 ·No. 5652 ·1978-03-30 ·Pages 423-8

Küpper H, Sekiya T, Rosenberg M, Egan J, Landy A

Abstract

A set of partially overlapping DNA restriction fragments that support promoter-dependent transcription of the tRNATyr1 gene of Escherichia coli has been used to study site-specific termination in vitro. Transcription termination occurs at a specific site 224-226 nucleotides beyond the end of the structural gene and is completely dependent on rho-factor. Certain features of this site suggest differences from other termination sites previously studied. A role for specific sequence recognition is suggested.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics Genes Nucleic Acid Precursors/genetics RNA, Bacterial/genetics RNA, Transfer/genetics Rho Factor/genetics Suppression, Genetic Transcription Factors/genetics Transcription, Genetic Tyrosine
Chemicals
DNA, Bacterial Nucleic Acid Precursors RNA, Bacterial Rho Factor Transcription Factors Tyrosine RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Küpper H
Sekiya T
Rosenberg M
Egan J
Landy A
References (30)
30 references, click to expand
  1. Isolation and sequence determination of the 3'-terminal regions of isotopically labelled RNA molecules.
    Nucleic Acids Res. 1974 May;1(5):653-71 PMID: 10793747
  2. A two-dimensional fractionation procedure for radioactive nucleotides.
    J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
  3. Factor stimulating transcription by RNA polymerase.
    Nature. 1969 Jan 4;221(5175):43-6 PMID: 4882047
  4. Termination factor for RNA synthesis.
    Nature. 1969 Dec 20;224(5225):1168-74 PMID: 4902144
  5. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
    Eur J Biochem. 1969 Dec;11(2):395-9 PMID: 4902612
  6. Duplicate genes for tyrosine transfer RNA in Escherichia coli.
    J Mol Biol. 1970 Jan 14;47(1):1-13 PMID: 4905086
  7. Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid.
    J Biol Chem. 1971 Aug 25;246(16):5120-39 PMID: 4936723
  8. Tyrosine tRNA precursor molecule polynucleotide sequence.
    Nat New Biol. 1971 Sep 8;233(36):35-9 PMID: 4938965
  9. In vitro synthesis of tRNA Tyr precursors and their conversion to 4S RNA.
    Nat New Biol. 1971 Sep 15;234(50):198-201 PMID: 5288804
  10. RNA synthesis startpoints in bacteriophage lambda: are the promoter and operator transcribed?
    Nat New Biol. 1972 Jun 21;237(77):227-32 PMID: 4556643
  11. Studies on termination of in vitro ribonucleic acid synthesis by rho factor.
    J Biol Chem. 1972 Sep 10;247(17):5637-45 PMID: 4560202
  12. Bacteriophage phi 80-induced low molecular weight RNA.
    Arch Biochem Biophys. 1972 Sep;152(1):152-65 PMID: 4342104
  13. The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585-9 PMID: 4587256
  14. Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides.
    J Mol Biol. 1973 Jun 25;77(2):255-77 PMID: 4587752
  15. DNA fragments carrying genes for tRNATyrI.
    Nature. 1974 Jun 21;249(459):738-42 PMID: 4545997
  16. Transcriptional control of in vitro tRNA-Tyr synthesis.
    Biochemistry. 1974 Sep 10;13(19):4058-62 PMID: 4607307
  17. Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8 PMID: 1093168
  18. New features of the regulation of the tryptophan operon.
    Science. 1975 Jul 4;189(4196):22-6 PMID: 1094538
  19. Termination of transcription in bacteriophage lambda. Heterogeneous, 3'-terminal oligo-adenylate additions and the effects of rho factor.
    J Biol Chem. 1975 Jun 25;250(12):4755-64 PMID: 1095576
  20. In vitro synthesis of transfer RNA. II. Identification of required enzymatic activities.
    J Biol Chem. 1975 Aug 25;250(16):6248-55 PMID: 1099090
  21. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  22. Promoter-dependent transcription of tRNAITyr genes using DNA fragments produced by restriction enzymes.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4754-8 PMID: 1107999
  23. Determination of nucleotide sequences beyond the sites of transcriptional termination.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):717-21 PMID: 1062781
  24. Nucleotide sequence of 5 S ribosomal RNA precursor from Bacillus subtilis.
    J Biol Chem. 1976 Jun 10;251(11):3480-8 PMID: 179998
  25. Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1959-63 PMID: 132662
  26. Transcription in vitro of bacteriophage lambda 4S RNA: studies on termination and rho protein.
    Nucleic Acids Res. 1977 Apr;4(4):827-42 PMID: 325526
  27. Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4365-9 PMID: 337297
  28. Computer analysis of nucleic acid regulatory sequences.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4401-5 PMID: 270683
  29. The relationship between function and DNA sequence in an intercistronic regulatory region in phage lambda.
    Nature. 1978 Mar 30;272(5652):414-23 PMID: 634366
  30. Structure and function of the cy control region of bacteriophage lambda.
    J Mol Biol. 1980 Apr;138(2):209-30 PMID: 6447790
Article Info
Journal
Nature
Abbr.
Nature
ISSN
0028-0836
DOI
10.1038/272423a0
Published
1978-03-30
Pages
423-8
Language
English
Region
England
NLM ID
0410462
PMCID
PMC1994828
Subset
IM
Grants
NIGMS NIH HHS · R01 GM033928 · United States
NIGMS NIH HHS · R01 GM062723 · United States
Databases
GENBANK
J01720, K01197, K01198, K01217, K01300, M10704
External Links
PubMed source DOI: 10.1038/272423a0 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2