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PMID: 1062781 Published · ppublish English Journal Article

Determination of nucleotide sequences beyond the sites of transcriptional termination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 3 ·1976-03-00 ·Pages 717-21

Rosenberg M, de Chrombrugghe B, Musso R

Abstract

A procedure is described by which a discrete high-molecular-weight RNA transcription product can be used as a primer by DNA polymerase (DNA nucleotidyltransferase; EC 2.7.7.7; deoxynucleoside triphosphate: DNA deoxynucleotidyltransferase) for determining nucleic acid sequence in the template DNA beyond the 3'-terminus of the transcript. This procedure is applied to two lambda phage transcripts, the 4S "oop" RNA [Short l-strand RNA transcript from the region of origin of replication (ori) and the 6S RNA. Sequences of 35 and 19 nucleotides, respectively, following the sites at which these two transcripts terminate, are determined. Little structural homology is apparent in the template DNA beyond the 3'-ends of these two transcripts. The lack of homology suggests that this region might not be important to the termination process.

MeSH Terms
Base Sequence Coliphages DNA Nucleotidyltransferases/metabolism DNA, Viral/analysis RNA, Viral/metabolism Transcription, Genetic
Chemicals
DNA, Viral RNA, Viral DNA Nucleotidyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rosenberg M
de Chrombrugghe B
Musso R
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.3.717
Published
1976-03-00
Pages
717-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335989
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.3.717 PMC Full Text View journal details
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