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PMID: 132662 Published · ppublish English Journal Article

Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 1959-63

Das A, Court D, Adhya S

Abstract

Polarity suppressor mutants that are conditional lethal for growth have been isolated in E. coli K12. The mutations map between the ilv and cya loci of the E. coli chromosome. Rho factor isolated from one of these ts mutants does not show transcription termination activity at any temperature tested; however, it is found to be temperature sensitive for its poly(C)-dependent ATPase activity. Unlike the previously known polarity suppressor mutants (suA and psu), the rho mutation suppresses all types of polarity. Other interesting properties of these mutants include ultraviolet sensitivity, recombination deficiency, and decreased ability to lysogenize temperate phages lambda and P1. Our results suggest that rho has an essential function in the growth and normal physiology of cells. The rho(ts) mutant allows the growth of phage lambda defective in the N gene. This result supports the model that N gene product prevents transcription termination by antagonizing rho activity.

MeSH Terms
Adenosine Triphosphatases/metabolism Chromosome Mapping Coliphages/growth & development DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology,radiation effects Lysogeny Mutation Operon Radiation Effects Recombination, Genetic Temperature Transcription, Genetic Ultraviolet Rays
Chemicals
DNA-Directed RNA Polymerases Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Das A
Court D
Adhya S
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.6.1959
Published
1976-06-00
Pages
1959-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430427
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.6.1959 PMC Full Text View journal details
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