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PMID: 1107999 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Promoter-dependent transcription of tRNAITyr genes using DNA fragments produced by restriction enzymes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 12 ·1975-12-00 ·Pages 4754-8

Küpper H, Contreras R, Landy A, Khorana HG

Abstract

Two DNA fragments prepared from the transducing bacteriophage strains ø80psuIII+ and ø80hpsuIII+,- by digestion with restriction enzymes contain one tyrosine tRNA gene (suIII+) and two tyrosine tRNA genes (suIII+, su-) in tandem, respectively, a single promoter in both cases, and some additional DNA regions at the two ends of both. Using these fragments, we have studied characteristics of the promoter-dependent transcription of the tyrosine tRNA genes. The promoter-dependent transcripts were shown to correspond to the expected tRNA precursors. Exposure of the transcript from the single gene fragment to an S100 extract from Escherichia coli gave, via intermediates, 4S material which was active in enzymatically accepting tyrosine and contained some modified bases.

MeSH Terms
Adenosine Triphosphate/pharmacology Base Sequence Cytosine Nucleotides/pharmacology DNA Restriction Enzymes/metabolism DNA, Bacterial/metabolism Endonucleases/metabolism Escherichia coli/metabolism Genes Guanosine Triphosphate/pharmacology Heparin/pharmacology Oligonucleotides/pharmacology RNA Nucleotidyltransferases/metabolism RNA, Transfer/metabolism Rifampin/pharmacology Transcription, Genetic/drug effects Tyrosine
Chemicals
Cytosine Nucleotides DNA, Bacterial Oligonucleotides Tyrosine Guanosine Triphosphate Adenosine Triphosphate Heparin RNA, Transfer RNA Nucleotidyltransferases Endonucleases DNA Restriction Enzymes Rifampin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Küpper H
Contreras R
Landy A
Khorana H G
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.12.4754
Published
1975-12-00
Pages
4754-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388809
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.12.4754 PMC Full Text View journal details
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