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PMID: 1108001 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A method for the isolation of specific tRNA precursors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 12 ·1975-12-00 ·Pages 4790-4

Vögeli G, Grosjean H, Söll D

Abstract

tRNA affinity chromatography, based on complex formation between tRNAs with complementary anticodons, has been applied to the isolation of specific tRNA precursors. When [32P]RNA, isolated from an Escherichia coli strain containing a thermolabile ribonuclease P, was chromatographed on resin-bound yeast phenylalanine tRNA, precursor tRNAGlu (possessing the complementary anticodon) was specifically retained. Likewise, precursor tRNAPhe was isolated from a column of resin-bound E. coli glutamate tRNA. Both precursor tRNAs isolated were monomeric and may be processed products of an originally larger RNA precursor. Both tRNA precursors contain additional nucleotides beyond the 5'-end of the mature tRNA and have all modified bases found in mature tRNA. The method can be extended to isolate other tRNA precursors by affinity chromatography with different tRNAs. Since the principle of complementary anticodon interaction is not restricted to any particular organism, specific precursor tRNAs from other sources may also be isolated in this way.

MeSH Terms
Base Sequence Chromatography, Affinity Escherichia coli/analysis Glutamates Oligoribonucleotides/analysis Pancreas/enzymology RNA, Transfer/analysis,isolation & purification Ribonucleases
Chemicals
Glutamates Oligoribonucleotides RNA, Transfer Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vögeli G
Grosjean H
Söll D
References (25)
25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.12.4790
Published
1975-12-00
Pages
4790-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388817
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.12.4790 PMC Full Text View journal details
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