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PMID: 349554 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Specific binding of tRNAMet to 23S rRNA of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 3 ·1978-03-00 ·Pages 1071-5

Dahlberg JE, Kintner C, Lund E

Abstract

tRNAMetf binds to 23S rRNA of Escherichia coli, forming a complex with a melting temperature of 75 degrees (in 0.6 M NaCl). The regions within the RNAs that bind to each other have been isolated and their nucleotide sequences have been determined. The interacting region in tRNAMetf is 17 nucleotides long, extending from G5 in the acceptor stem to D21 (D = 5.6-dihydrouridine) in the D loop. The sequence in 23S rRNA is complementary to that sequence except for an extra Up in the middle and allowing a Gp.D base pair. We propose that association of these two sequences may play a role in initiation of protein synthesis by tRNAMetf. In addition, part of this sequence in 23S rRNA may also stabilize tRNA binding to the ribosome during elongation of nascent polypeptides.

MeSH Terms

Base Sequence Escherichia coli N-Formylmethionine Protein Biosynthesis RNA, Bacterial/genetics,metabolism RNA, Ribosomal/genetics,metabolism RNA, Transfer/genetics,metabolism

Chemicals

RNA, Bacterial RNA, Ribosomal N-Formylmethionine RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dahlberg J E

Kintner C

Lund E

References (18)

18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.3.1071

Published

1978-03-00

Pages

1071-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC411410

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.3.1071 PMC Full Text View journal details

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