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PMID: 6275359 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structure and sequence of the mitochondrial 20S rRNA and tRNA tyr gene of Paramecium primaurelia.

Nucleic acids research ·Vol. 9 ·No. 23 ·1981-12-11 ·Pages 6391-406

Seilhamer JJ, Cummings DJ

Abstract

The sequence and structure of the large (20s) mitochondrial (mt) rRNA gene and flanking regions from Paramecium primaurelia have been determined. The gene contains two regions of strong homology with other large mt rRNAs: one 44-base region near the 5' end and a 321-base region near the 3' end. Another region of strong homology to both ends of E. coli 23s RNA exists at loci consistent with these regions. The Paramecium gene appears to be 2204 bases in length and contains slightly more homology to E. coli rRNA than its mammalian or fungal counterparts. The gene, located about 1200 bp from the replicative terminal end of the linear mt DNA, is transcribed in the same polarity as replication. Previous R-looping studies detected no large introns within the gene. Here we describe sequences resembling degenerate rRNAs, one of which could represent a small intron. A tRNA tyr gene was found on the same DNA strand, 127 bp downstream from the large rRNA presumptive 3' end. The tRNA is flanked on both sides by short DNA regions of approximately 90% A + T content.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes DNA, Mitochondrial/genetics Genes Nucleic Acid Conformation Paramecium/genetics RNA, Ribosomal/genetics RNA, Transfer/genetics
Chemicals
DNA, Mitochondrial RNA, Ribosomal RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Seilhamer J J
Cummings D J
References (39)
39 references, click to expand
  1. The transcription termination site of the ribosomal RNA operon in yeast.
    Nucleic Acids Res. 1980 Nov 25;8(22):5179-92 PMID: 6258138
  2. The nucleotide sequence of the large ribosomal RNA gene and the adjacent tRNA genes from rat mitochondria.
    Nucleic Acids Res. 1981 Aug 25;9(16):4139-48 PMID: 6913863
  3. Methylated regions of hamster mitochondrial ribosomal RNA: structural and functional correlates.
    Nucleic Acids Res. 1981 Jan 24;9(2):323-37 PMID: 6782552
  4. The 3' terminus of the large ribosomal subunit ("17S") RNA from hamster mitochondria is ragged and oligoadenylated.
    Cell. 1981 Jan;23(1):271-8 PMID: 7214525
  5. Sequence and organization of the human mitochondrial genome.
    Nature. 1981 Apr 9;290(5806):457-65 PMID: 7219534
  6. Altered ribosomal RNA genes in mitochondria from mammalian cells with chloramphenicol resistance.
    Nature. 1981 Apr 16;290(5807):607-8 PMID: 7219548
  7. Transcriptional initiation and 5' termini of yeast mitochondrial RNA.
    J Biol Chem. 1981 May 25;256(10):5226-32 PMID: 6262322
  8. Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver.
    Biochim Biophys Acta. 1966 Dec 21;129(3):554-62 PMID: 5337973
  9. Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.
    Nature. 1968 Mar 16;217(5133):1019-24 PMID: 5643523
  10. Mitochondrial nucleic acids.
    Annu Rev Biochem. 1972;41:333-76 PMID: 4563438
  11. Structure and replication of mitochondrial DNA from Paramecium aurelia.
    J Mol Biol. 1975 Oct 5;97(4):593-609 PMID: 1185783
  12. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  13. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  14. Mitochondrial DNA replication in Paramecium aurelia. Cross-linking of the initiation end.
    J Mol Biol. 1977 Jan 15;109(2):327-44 PMID: 839545
  15. The mitochondrial genome of yeast.
    Cell. 1978 Nov;15(3):705-23 PMID: 153200
  16. Interesting and unusual features in the sequence of Neurospora crassa mitochondrial tyrosine transfer RNA.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):717-21 PMID: 154673
  17. Organization of tRNA and rRNA genes in N. crassa mitochondria: intervening sequence in the large rRNA gene and strand distribution of the RNA genes.
    Cell. 1979 Jul;17(3):583-95 PMID: 157815
  18. Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):201-4 PMID: 6153795
  19. Physical map of Aspergillus nidulans mitochondrial genes coding for ribosomal RNA: an intervening sequence in the large rRNA cistron.
    Mol Gen Genet. 1980 Feb;177(3):389-97 PMID: 6246396
  20. Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the omega and rib-1 loci.
    Cell. 1980 May;20(1):185-97 PMID: 6156002
  21. Splice point sequence and transcripts of the intervening sequence in the mitochondrial 21S ribosomal RNA gene of yeast.
    Cell. 1980 May;20(1):207-14 PMID: 6993009
  22. Anatomy of mitochondrial DNA from Paramecium aurelia.
    Mol Gen Genet. 1980;178(3):499-510 PMID: 6248732
  23. Distinctive sequence of human mitochondrial ribosomal RNA genes.
    Nature. 1980 Jul 31;286(5772):460-7 PMID: 6157106
  24. Novel features in the genetic code and codon reading patterns in Neurospora crassa mitochondria based on sequences of six mitochondrial tRNAs.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3159-63 PMID: 6447871
  25. Different pattern of codon recognition by mammalian mitochondrial tRNAs.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3164-6 PMID: 6932013
  26. Codon recognition rules in yeast mitochondria.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3167-70 PMID: 6997870
  27. Precise localization and nucleotide sequence of the two mouse mitochondrial rRNA genes and three immediately adjacent novel tRNA genes.
    Cell. 1980 Nov;22(1 Pt 1):157-70 PMID: 7428037
  28. Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S--23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions.
    J Biol Chem. 1980 Nov 25;255(22):10997-1003 PMID: 6253497
  29. The transcription map of human mitochondrial DNA implicates transfer RNA excision as a major processing event.
    J Biol Chem. 1980 Dec 10;255(23):11599-606 PMID: 6254975
  30. Sequence homologies between Escherichia coli and chloroplast ribosomal DNA as seen by heteroduplex analysis.
    J Mol Biol. 1980 Sep 15;142(2):247-61 PMID: 7003161
  31. Homologies among ribosomal RNA and messenger RNA genes in chloroplasts, mitochondria and E. coli.
    Mol Gen Genet. 1980;179(3):539-45 PMID: 7003301
  32. The tRNA genes punctuate the reading of genetic information in human mitochondrial DNA.
    Cell. 1980 Nov;22(2 Pt 2):393-403 PMID: 7448867
  33. Transcripts and processing patterns for the ribosomal RNA and transfer RNA region of Neurospora crassa mitochondrial DNA.
    J Biol Chem. 1981 Feb 25;256(4):2027-34 PMID: 6450763
  34. Mitochondrial DNA of chloramphenicol-resistant mouse cells contains a single nucleotide change in the region encoding the 3' end of the large ribosomal RNA.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3789-93 PMID: 6943583
  35. Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.
    Cell. 1981 Jul;25(1):203-13 PMID: 7023695
  36. The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA.
    Nucleic Acids Res. 1981 Jun 25;9(12):2853-69 PMID: 7024906
  37. Sequence homologies between eukaryotic 5.8S rRNA and the 5' end of prokaryotic 23S rRNa: evidences for a common evolutionary origin.
    Nucleic Acids Res. 1981 Jun 25;9(12):2913-32 PMID: 7024907
  38. Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes.
    Nucleic Acids Res. 1981 Jul 24;9(14):3287-306 PMID: 7024913
  39. Mitochondrial tRNA gene clusters in Aspergillus nidulans: organization and nucleotide sequence.
    Cell. 1981 Feb;23(2):625-33 PMID: 7008953
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.23.6391
Published
1981-12-11
Pages
6391-406
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327611
Subset
IM
Grants
NIA NIH HHS · 1-F32-AG-05221-01 · United States
NIGMS NIH HHS · GM 21948 · United States
Databases
GENBANK
K00634
External Links
PubMed source DOI: 10.1093/nar/9.23.6391 PMC Full Text View journal details
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