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PMID: 2838457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence analysis of termini of conjugative transposon Tn916.

Journal of bacteriology ·Vol. 170 ·No. 7 ·1988-07-00 ·Pages 3046-52

Clewell DB, Flannagan SE, Ike Y, Jones JM, Gawron-Burke C

Abstract

Transposon Tn916 is a 16.4-kilobase, broad-host-range, conjugative transposon originally identified on the chromosome of Enterococcus (Streptococcus) faecalis DS16. Its termini have been sequenced along with the junction regions for two different insertions. The ends were found to contain imperfect inverted repeat sequences with identity at 20 of 26 nucleotides. Further in from the ends, imperfect directly repeated sequences were present, with 24 of 27 nucleotides matching. The transposon junction regions contained homologous segments but of a nature not consistent with a direct duplication of the target sequence. Within the right terminus was a potential outwardly reading promoter. Tn916 is believed to transpose via an excision-insertion mechanism; based on the analyses of the termini, as well as two target sequences (before insertion and after excision), a possible model is suggested.

MeSH Terms
Base Sequence Cloning, Molecular Conjugation, Genetic DNA Transposable Elements Enterococcus faecalis/genetics Escherichia coli/genetics Molecular Sequence Data Plasmids Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Clewell D B
Department of Oral Biology, School of Dentistry, University of Michigan, Ann Arbor 48109.
Flannagan S E
Ike Y
Jones J M
Gawron-Burke C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.7.3046-3052.1988
Published
1988-07-00
Pages
3046-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211247
Subset
IM
Grants
NIAID NIH HHS · AI-10318 · United States
NIDCR NIH HHS · DE-02731 · United States
Databases
GENBANK
M20864, M22645
External Links
PubMed source DOI: 10.1128/jb.170.7.3046-3052.1988 PMC Full Text View journal details
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