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PMID: 3027505 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transposable multiple antibiotic resistance in Streptococcus pneumoniae.

Molecular & general genetics : MGG ·Vol. 205 ·No. 2 ·1986-11-00 ·Pages 291-7

Courvalin P, Carlier C

Abstract

A mobile genetic element, designated Tn1545, was detected in the chromosome of Streptococcus pneumoniae BM4200, a clinical isolate multiply resistant to antibiotics. The 25.3 kb element conferred resistance to kanamycin and structurally related aminoglycosides by synthesis of a 3'-aminoglycoside phosphotransferase type III (aphA-3), to macrolide-lincosamide-streptogramin B-type antibiotics (ermAM), and to tetracycline (tetM). Tn1545 was self-transferable to a recombination deficient S. faecalis strain where it was able to transpose to various sites, induce insertional mutations and was apparently cleanly excised. The element also conjugated to and transposed to the chromosome of S. faecalis, S. lactis, S. diacetylactis, S. cremoris, S. sanguis, Staphylococcus aureus, and Listeria monocytogenes. The properties of the conjugative transposon Tn1545 could account for the sudden emergence, rapid dissemination, and stabilisation of multiple resistance to antibiotics in S. pneumoniae in the absence of plasmids.

MeSH Terms
Aminoglycosides/pharmacology Anti-Bacterial Agents/pharmacology Bacteria/genetics Conjugation, Genetic DNA Transposable Elements Drug Resistance, Microbial Phenotype R Factors Streptococcus pneumoniae/drug effects,genetics
Chemicals
Aminoglycosides Anti-Bacterial Agents DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Courvalin P
Carlier C
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40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00430441
Published
1986-11-00
Pages
291-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00430441
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