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PMID: 7216474 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of plasmids and plasmid-associated determinants of Yersinia enterocolitica pathogenesis.

Infection and immunity ·Vol. 31 ·No. 2 ·1981-02-00 ·Pages 775-82

Portnoy DA, Moseley SL, Falkow S

Abstract

Yersinia enterocolitica isolates harboring a particular species of plasmid deoxyribonucleic acid showed a high degree of lethality for gerbils and caused the detachment of HEp-2 tissue cell monolayers. Strains cured of their plasmid deoxyribonucleic acid showed loss of these properties. However, invasiveness of HEp-2 cells was shown not to be a plasmid-mediated property. The expression of plasmid-associated properties, including at least three major outer membrane polypeptides, occurred during growth at 37 but not at 25 degrees C and was related to the concentration of calcium in the growth medium. The plasmid species associated with these properties ranged in molecular mass from 40 x 10(6) to 48 x 10(6) daltons and comprised a family of related plasmids.

MeSH Terms
Animals Bacterial Proteins/genetics Cell Adhesion Cells, Cultured DNA, Bacterial/genetics Disease Models, Animal Gerbillinae Membrane Proteins/genetics Plasmids Yersinia/genetics,pathogenicity
Chemicals
Bacterial Proteins DNA, Bacterial Membrane Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Portnoy D A
Moseley S L
Falkow S
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26 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.31.2.775-782.1981
Published
1981-02-00
Pages
775-82
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC351377
Subset
IM
Grants
NIAID NIH HHS · AI07149-02 · United States
External Links
PubMed source DOI: 10.1128/iai.31.2.775-782.1981 PMC Full Text View journal details
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