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PMID: 3035335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tn1545: a conjugative shuttle transposon.

Molecular & general genetics : MGG ·Vol. 206 ·No. 2 ·1987-02-00 ·Pages 259-64

Courvalin P, Carlier C

Abstract

Tn1545, from Streptococcus pneumoniae BM4200, confers resistance to kanamycin (aphA-3), erythromycin (ermAM) and tetracycline (tetM). The 25.3 kb element is self-transferable to various Gram-positive bacterial genera where it transposes. Tn1545 was cloned in its entirety in the recombination deficient Escherichia coli HB101 where it was unstable. The three resistance genes aphA-3, ermAM and tetM were expressed but were not transferable to other E. coli cells. Tn1545 transposed from the hybrid plasmid to multiple sites of the chromosome of its new host. The element re-transposed, at a frequency of 5 X 10(-9), from the chromosome to various sites of a conjugative plasmid where it could be lost by apparently clean excision. The element transformed and transposed to the chromosome of Bacillus subtilis. The properties of the conjugative shuttle transposon Tn1545 may account for the recent emergence of genes from Gram-positive bacteria in Gram-negative organisms.

MeSH Terms
Anti-Bacterial Agents/pharmacology Conjugation, Genetic DNA Transposable Elements Drug Resistance, Microbial Escherichia coli/drug effects,genetics Genes, Bacterial Phenotype Streptococcus pneumoniae/drug effects,genetics
Chemicals
Anti-Bacterial Agents DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Courvalin P
Carlier C
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333582
Published
1987-02-00
Pages
259-64
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00333582
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