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PMID: 6995434 Published · ppublish English Journal Article

Conjugative transfer of multiple antibiotic resistance markers in Streptococcus pneumoniae.

Journal of bacteriology ·Vol. 143 ·No. 1 ·1980-07-00 ·Pages 313-20

Buu-Hoï A, Horodniceanu T

Abstract

Two antibiotic-resistant isolates of Streptococcus pneumoniae were investigated for conjugative transfer of their drug resistance markers into streptococcal (groups B and D) and pneumococcal (encapsulated and non-encapsulataed) recipients. Of these, 7 wild-type donor pneumococci transferred all their resistance markers (except Pc [penicillin], Su [sulfonamide], and Tp [trimethoprim]) into group D Streptococcus and non-encapsulated S. pneumoniae recipients at a low frequency (10(-5) to 10(-6)). The resistance markers transferred were Tc (tetracycline); Tc and Cm (chloramphenicol); Tc and MLS (macrolides, lincosamides, and streptogramin B); Tc, MLS, Km (kanamycin), and Cm. The transconjugants obtained retransferred their resistance markers into appropriate streptococcal or pneumococcal recipients or both. The resistance markers of streptococcal transconjugants could not be cured by chemical agents. All attempts to detect extra-chromosomal deoxyribonucleic acid from pneumococcal or streptococcal transconjugants were unsuccessful. The molecular weight of a streptococcal conjugative R plasmid (pIP501) was investigated after transfer into the non-encapsulated S. pneumoniae recipient and was found to be similar to that of the wild-type group B Streptococcus host (20 x 10(6)).

MeSH Terms
Acridine Orange/pharmacology Anti-Bacterial Agents/pharmacology Bacteriophages/growth & development Conjugation, Genetic Deoxyribonucleases/pharmacology Drug Resistance, Microbial Ethidium/pharmacology Genetic Markers Plasmids Streptococcus/genetics Streptococcus agalactiae/genetics Streptococcus pneumoniae/drug effects,genetics
Chemicals
Anti-Bacterial Agents Genetic Markers Deoxyribonucleases Ethidium Acridine Orange
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Buu-Hoï A
Horodniceanu T
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31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.143.1.313-320.1980
Published
1980-07-00
Pages
313-20
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294235
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.143.1.313-320.1980 PMC Full Text View journal details
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