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PMID: 2823065 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the ends of the conjugative shuttle transposon Tn1545.

Molecular & general genetics : MGG ·Vol. 209 ·No. 1 ·1987-08-00 ·Pages 110-5

Caillaud F, Courvalin P

Abstract

The conjugative shuttle transposon Tn1545 from Streptococcus pneumoniae transposes in various gram-positive bacterial genera following self-transfer and in Escherichia coli after cloning. Analysis of the junction fragments and of the targets before insertion and after excision of the element by DNA hybridization and sequencing indicated that Tn1545 (1) is not flanked by terminal repeated sequences in either direct or opposite orientation, (2) is flanked, in an asymmetric fashion, by terminal variable base pairs, one at the left and three at the right of the element, (3) inserts in a target DNA consensus sequence, (4) does not generate duplication of the target DNA upon insertion, and (5) excises precisely.

MeSH Terms
Base Sequence Cloning, Molecular Conjugation, Genetic DNA Restriction Enzymes DNA Transposable Elements Enterococcus faecalis/genetics Escherichia coli/genetics Nucleic Acid Hybridization Plasmids Streptococcus pneumoniae/genetics
Chemicals
DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Caillaud F
Unité des Agents Antibactériens, CNRS U.A. 271, Institut Pasteur, Paris, France.
Courvalin P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00329844
Published
1987-08-00
Pages
110-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00329844
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